中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Determination of DL-tetrahydropalmatine in Corydalis yanhusuo by L-tetrahydropalmatine imprinted monolithic column coupling with reversed-phase high performance liquid chromatography
作者: Ou, JJ;  Kong, L;  Pan, CS;  Su, XY;  Lei, XY;  Zou, HF
通讯作者: 邹汉法
关键词: molecularly imprinted polymer ;  monolithic column ;  tetrahydropalmatine ;  chiral separation ;  on-line ;  solid-phase extraction ;  Corydalis yanhusuo
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2006-06-09
DOI: 10.1016/j.chroma.2006.03.084
卷: 1117, 期:2, 页:163-169
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A method for direct determination of DL-tetrahydropalmatine (DL-THP) in Corydalis yanhusuo, a traditional Chinese herb, by L-THP imprinted monolithic precolumn on-line/off-line coupling with reversed-phase high performance liquid chromatography (RP-HPLC) was developed. The L-THP imprinted monolithic column has been prepared by in situ polymerization using methacrylic acid (MAA) and ethylene dimethacrylate (EDMA) as functional monomer and cross-linker, respectively. With the optimization of chromatographic conditions, such as mobile phase composition, flow rate, column temperature and sample loading, for the separation of enantiomer, DL-THP was base-line separated on the MIP. The imprinted monolithic column was used as a precolumn for fractionation of the C. yanhusuo extract. Both the non-retained and retained fractions were separated by RP-HPLC. Meanwhile, the D-THP and L-THP can be detected in the non-retained and retained fractions, respectively. Additionally, direct determination of L-THP using molecularly imprinted monolith on-line coupling with a reversed-phase column was acquired. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: GROWTH-FACTOR RECEPTOR ;  ENVIRONMENTAL WATER ;  MASS-SPECTROMETRY ;  STATIONARY PHASES ;  ORGANIC MODIFIER ;  EXTRACTION ;  POLYMERS ;  SEPARATION ;  SAMPLES ;  ENANTIOMERS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000238005800007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/93729
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Ou, JJ,Kong, L,Pan, CS,et al. Determination of DL-tetrahydropalmatine in Corydalis yanhusuo by L-tetrahydropalmatine imprinted monolithic column coupling with reversed-phase high performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1117(2):163-169.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Ou, JJ]'s Articles
 [Kong, L]'s Articles
 [Pan, CS]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Ou, JJ]‘s Articles
 [Kong, L]‘s Articles
 [Pan, CS]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace