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题名: Iminodiacetic acid derivatized porous silicon as a matrix support for sample pretreatment and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis
作者: Xu, SY;  Zhou, HJ;  Pan, CS;  Fu, Y;  Zhang, Y;  Li, X;  Ye, ML;  Zou, HF
通讯作者: 邹汉法
刊名: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表日期: 2006
DOI: 10.1002/rcm.2493
卷: 20, 期:11, 页:1769-1775
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Analytical ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Spectroscopy
英文摘要: Iminodiacetic acid (IDA)-1,2-epoxy-9-decene has been synthesized and covalently linked to the surface of porous silicon wafer through a photochemical reaction. The negatively charged carboxylic acid groups on the porous silicon wafer are capable of binding oppositely charged species from sample solutions through electrostatic interactions. This allows the removal of contaminants prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) by simply washing the porous silicon surface. The carboxylic acid end groups on porous silicon can be used to selectively bind and concentrate target species in sample solutions. Furthermore, Fe3+-IDA-derivatized porous silicon was prepared to specifically and effectively concentrate phosphopeptides from the tryptic digests of phosphoproteins, followed by MALDI-MS analysis. Copyright (c) 2006 John Wiley & Sons, Ltd.
关键词[WOS]: MALDI MS ANALYSIS ;  AFFINITY-CHROMATOGRAPHY ;  PROTEIN SAMPLES ;  PHOSPHORIC-ACID ;  IDENTIFICATION ;  SURFACES ;  ENZYME ;  PHOSPHOPEPTIDES ;  NANOPARTICLES ;  PERFORMANCE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000237821200023
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/93731
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Grad Sch, Dalian 116011, Peoples R China

Recommended Citation:
Xu, SY,Zhou, HJ,Pan, CS,et al. Iminodiacetic acid derivatized porous silicon as a matrix support for sample pretreatment and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2006,20(11):1769-1775.
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