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题名: Parallel analysis of biomolecules on a microfabricated capillary array chip
作者: Shen, Z;  Liu, XJ;  Long, ZC;  Liu, DY;  Ye, NN;  Qin, JH;  Dai, ZP;  Lin, BC
通讯作者: 林炳承
关键词: biological analysis ;  capillary array electrophoresis ;  microfluidic chip
刊名: ELECTROPHORESIS
发表日期: 2006-03-01
DOI: 10.1002/elps.200500689
卷: 27, 期:5-6, 页:1084-1092
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: This paper focused on a self-developed microfluiclic array system with microfabricated capillary array electrophoresis (mu-CAE) chip for parallel chip electrophoresis of biomolecules. The microfluiclic array layout consists of two common reservoirs coupled to four separation channels connected to sample injection channel on the soda-lime glass substrate. The excitation scheme for distributing a 20 mW laser beam to separation channels in an array is achieved. Under the control of program, the sample injection and separation in multichannel can be achieved through six high-voltage modules' output. A CCD camera was used to monitor electrophoretic separations simultaneously in four channels with LIF detection, and the electropherograms can be plotted directly without reconstruction by additional software. Parallel multichannel electrophoresis of series biomolecules including amino acids, proteins, and nucleic acids was performed on this system and the results showed fine reproducibility.
关键词[WOS]: TOTAL ANALYSIS SYSTEMS ;  THROUGHPUT GENETIC-ANALYSIS ;  SAMPLE INTRODUCTION ;  ELECTROPHORESIS ;  DNA ;  MICROCHIP ;  IMMUNOASSAY ;  TECHNOLOGY ;  DEVICES ;  CELLS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000236511900018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/93869
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Shen, Z,Liu, XJ,Long, ZC,et al. Parallel analysis of biomolecules on a microfabricated capillary array chip[J]. ELECTROPHORESIS,2006,27(5-6):1084-1092.
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