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题名: Analysis of multiplex PCR fragments with PMMA microchip
作者: Liu, DY;  Zhou, XM;  Zhong, RT;  Ye, NN;  Chang, GH;  Xiong, W;  Mei, XD;  Lin, BC
通讯作者: 林炳承
关键词: microchip electrophoresis ;  microchip fabrication ;  multiplex PCR
刊名: TALANTA
发表日期: 2006-01-15
DOI: 10.1016/j.talanta.2005.04.064
卷: 68, 期:3, 页:616-622
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Microchip electrophoresis is a promising technique for analysis of bio-molecules. It has the advantages of fast analysis, high sensitivity, high resolution and low-cost of samples. Plastic chip has the potential of mass production for clinical use for its advantages in biocompatibility and low cost. In this work, the method for fabrication of poly(methyl methacrylate) (PMMA) chip was described, and conditions for DNA separation were investigated with the chip. The PMMA microchip was used for detection of multiplex PCR products of 18 and 36 cases with SARS and hepatitis B virus infection under optimized separation conditions. Microchip electrophoresis showed higher sensitivity, higher resolution and less time consumption when compared with gel electrophoresis. The microchip electrophoresis with PMMA chip provided a rapid, sensitive and reliable method for analysis of multiplex PCR products. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  MICROFLUIDIC CHIP ;  FABRICATION ;  DEVICES ;  DNA ;  MOLECULES ;  SYSTEM
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000234955400018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/93871
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Guangzhou First Municipal Peoples Hosp, Guangzhou Med Coll, Dept Clin Lab, Guangzhou 510500, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100000, Peoples R China
4.Chinese Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China

Recommended Citation:
Liu, DY,Zhou, XM,Zhong, RT,et al. Analysis of multiplex PCR fragments with PMMA microchip[J]. TALANTA,2006,68(3):616-622.
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