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Analysis of multiplex PCR fragments with PMMA microchip
Liu, DY; Zhou, XM; Zhong, RT; Ye, NN; Chang, GH; Xiong, W; Mei, XD; Lin, BC; Lin BC(林炳承); Lin BC(林炳承)
关键词Microchip Electrophoresis Microchip Fabrication Multiplex Pcr
刊名TALANTA
2006-01-15
DOI10.1016/j.talanta.2005.04.064
68期:3页:616-622
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]CAPILLARY-ELECTROPHORESIS ; MICROFLUIDIC CHIP ; FABRICATION ; DEVICES ; DNA ; MOLECULES ; SYSTEM
英文摘要Microchip electrophoresis is a promising technique for analysis of bio-molecules. It has the advantages of fast analysis, high sensitivity, high resolution and low-cost of samples. Plastic chip has the potential of mass production for clinical use for its advantages in biocompatibility and low cost. In this work, the method for fabrication of poly(methyl methacrylate) (PMMA) chip was described, and conditions for DNA separation were investigated with the chip. The PMMA microchip was used for detection of multiplex PCR products of 18 and 36 cases with SARS and hepatitis B virus infection under optimized separation conditions. Microchip electrophoresis showed higher sensitivity, higher resolution and less time consumption when compared with gel electrophoresis. The microchip electrophoresis with PMMA chip provided a rapid, sensitive and reliable method for analysis of multiplex PCR products. (c) 2005 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000234955400018
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被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/93871
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承); Lin BC(林炳承)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Guangzhou First Municipal Peoples Hosp, Guangzhou Med Coll, Dept Clin Lab, Guangzhou 510500, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100000, Peoples R China
4.Chinese Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China
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Liu, DY,Zhou, XM,Zhong, RT,et al. Analysis of multiplex PCR fragments with PMMA microchip[J]. TALANTA,2006,68(3):616-622.
APA Liu, DY.,Zhou, XM.,Zhong, RT.,Ye, NN.,Chang, GH.,...&林炳承.(2006).Analysis of multiplex PCR fragments with PMMA microchip.TALANTA,68(3),616-622.
MLA Liu, DY,et al."Analysis of multiplex PCR fragments with PMMA microchip".TALANTA 68.3(2006):616-622.
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