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题名: Optimization of the cell seeding density and modeling of cell growth and metabolism using the modified Gompertz model for microencapsulated animal cell culture
作者: Qi, WT;  Zhang, Y;  Ma, JA;  Guo, X;  Xie, YB;  Wang, W;  Ma, XJ
通讯作者: 马小军
关键词: microencapsulation ;  microcapsule ;  cell seeding density ;  modeling ;  cell culture
刊名: BIOTECHNOLOGY AND BIOENGINEERING
发表日期: 2006-04-05
DOI: 10.1002/bit.20782
卷: 93, 期:5, 页:887-895
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Cell microericapsulation is one of the promising strategies for the in vitro production of proteins or in vivo delivery of therapeutic products. In order to design and fabricate the optimized microencapsulated cell system, the Gompertz model was applied and modified to describe the growth and metabolism of microencapsulated cell, including substrate consumption and product formation. The Gompertz model successfully described the cell growth kinetics and the modified Gompertz models fitted the substrate consumption and product formation well. It was demonstrated that the optimal initial cell seeding density was about 4-5 x 10(6) cells/mL of microcapsule, in terms of the maximum specific growth rate, the glucose consumption potential and the product formation potential calculated by the Gompertz and modified Gompertz models. Modeling of cell growth and metabolism in microcapsules provides a guideline for optimizing the culture of microencapsulated cells. (c) 2005 Wiley Periodicals, Inc.
关键词[WOS]: MAMMALIAN-CELLS ;  CANCER GROWTH ;  LACTATE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000236592000007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97129
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Qi, WT,Zhang, Y,Ma, JA,et al. Optimization of the cell seeding density and modeling of cell growth and metabolism using the modified Gompertz model for microencapsulated animal cell culture[J]. BIOTECHNOLOGY AND BIOENGINEERING,2006,93(5):887-895.
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