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题名: Cytoskeletal reorganization and repolarization of hepatocarcinoma cells in APA microcapsule to mimic native tumor characteristics
作者: Wang, Q;  Li, SY;  Xie, YB;  Yu, WT;  Xiong, Y;  Ma, XJ;  Yuan, Q
通讯作者: 马小军
关键词: hepatocarcinoma cells ;  microcapsule ;  polarization ;  cytoskeleton ;  multicellular spheroid
刊名: HEPATOLOGY RESEARCH
发表日期: 2006-06-01
DOI: 10.1016/j.hepres.2006.03.003
卷: 35, 期:2, 页:96-103
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
英文摘要: Culture of human hepatocarcinoma cells in three-dimensional structure or spheroid represents a unique opportunity to recapitulate aspects of cell homeostasis and to better reflect the characteristics of native hepatic tumor. In this paper, human hepatocarcinoma SMMC-7721 cells were encapsulated in alginate-poly-L-lysine-alginate (APA) microcapsules and the behavior of the cells in the restricted three-dimensional culture system was investigated. The cells were polygonal-shaped, proliferating with high viability, and formed a single multicellular spheroid in microcapsule. During the assembly of the aggregate in microcapsule, cytoskeletal reorganization and repolorization was occurred, which was absent in cells cultured in monolayer. The in vivo-like cytoarchitecture was confirmed by the phalloidin-FITC labeling of F-actin filaments. The trabecular structure revealed by scanning electron microscope (SEM), and the bile canaliculus with multiple microvilli and tight junctions revealed by transmission electron microscope (TEM) was found in microencapsulated cells, which exhibited the ultrastructural characteristics of native hepatic tissue. It suggested that microcapsule culture provides an in vivo-like model which can be used for the study of the interactions of cell-cell and cell-microenvironment, and the mechanisms of invasion, metastasis and multicellular drug resistance of tumor cells. (c) 2006 Elsevier Ireland Ltd. All fights reserved.
关键词[WOS]: SPHEROID MODEL ;  GROWTH ;  MICROENCAPSULATION ;  GENERATION ;  MIGRATION ;  POLARITY ;  CULTURE ;  LIVER
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000238585800005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97137
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Wang, Q,Li, SY,Xie, YB,et al. Cytoskeletal reorganization and repolarization of hepatocarcinoma cells in APA microcapsule to mimic native tumor characteristics[J]. HEPATOLOGY RESEARCH,2006,35(2):96-103.
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