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Cytoskeletal reorganization and repolarization of hepatocarcinoma cells in APA microcapsule to mimic native tumor characteristics
Wang, Q; Li, SY; Xie, YB; Yu, WT; Xiong, Y; Ma, XJ; Yuan, Q; Ma XJ(马小军); Ma XJ(马小军)
关键词Hepatocarcinoma Cells Microcapsule Polarization Cytoskeleton Multicellular Spheroid
刊名HEPATOLOGY RESEARCH
2006-06-01
DOI10.1016/j.hepres.2006.03.003
35期:2页:96-103
收录类别SCI
文章类型Article
部门归属18
项目归属1802
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]SPHEROID MODEL ; GROWTH ; MICROENCAPSULATION ; GENERATION ; MIGRATION ; POLARITY ; CULTURE ; LIVER
英文摘要Culture of human hepatocarcinoma cells in three-dimensional structure or spheroid represents a unique opportunity to recapitulate aspects of cell homeostasis and to better reflect the characteristics of native hepatic tumor. In this paper, human hepatocarcinoma SMMC-7721 cells were encapsulated in alginate-poly-L-lysine-alginate (APA) microcapsules and the behavior of the cells in the restricted three-dimensional culture system was investigated. The cells were polygonal-shaped, proliferating with high viability, and formed a single multicellular spheroid in microcapsule. During the assembly of the aggregate in microcapsule, cytoskeletal reorganization and repolorization was occurred, which was absent in cells cultured in monolayer. The in vivo-like cytoarchitecture was confirmed by the phalloidin-FITC labeling of F-actin filaments. The trabecular structure revealed by scanning electron microscope (SEM), and the bile canaliculus with multiple microvilli and tight junctions revealed by transmission electron microscope (TEM) was found in microencapsulated cells, which exhibited the ultrastructural characteristics of native hepatic tissue. It suggested that microcapsule culture provides an in vivo-like model which can be used for the study of the interactions of cell-cell and cell-microenvironment, and the mechanisms of invasion, metastasis and multicellular drug resistance of tumor cells. (c) 2006 Elsevier Ireland Ltd. All fights reserved.
语种英语
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WOS记录号WOS:000238585800005
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被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/97137
专题中国科学院大连化学物理研究所
通讯作者Ma XJ(马小军); Ma XJ(马小军)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Wang, Q,Li, SY,Xie, YB,et al. Cytoskeletal reorganization and repolarization of hepatocarcinoma cells in APA microcapsule to mimic native tumor characteristics[J]. HEPATOLOGY RESEARCH,2006,35(2):96-103.
APA Wang, Q.,Li, SY.,Xie, YB.,Yu, WT.,Xiong, Y.,...&马小军.(2006).Cytoskeletal reorganization and repolarization of hepatocarcinoma cells in APA microcapsule to mimic native tumor characteristics.HEPATOLOGY RESEARCH,35(2),96-103.
MLA Wang, Q,et al."Cytoskeletal reorganization and repolarization of hepatocarcinoma cells in APA microcapsule to mimic native tumor characteristics".HEPATOLOGY RESEARCH 35.2(2006):96-103.
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