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K562 cell growth activity and metabolism characteristics in APA microencapsulated culture and modeling study
Ma, Juan; Qi, Wentao; Xie, Yubing; Wang, Wei; Yu, Weiting; Ma, Xiaojun; Ma XJ(马小军); Ma XJ(马小军)
关键词Microencapsulation Cell Activity Cell Growth And Metabolism Modeling
刊名JOURNAL OF BIOTECHNOLOGY
2006-09-01
DOI10.1016/j.jbiotec.2006.02.025
125期:2页:242-251
收录类别SCI
文章类型Article
部门归属18
项目归属1802
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]HUMAN FACTOR-IX ; MAMMALIAN-CELLS ; MICROCAPSULES ; EXPRESSION ; DELIVERY ; LACTATE ; ISLETS ; KIDNEY ; MICE
英文摘要Cell microencapsulation is likely to play a major role in cell and transplantation therapies in the next decade. The microcapsules provide a special microenvironment in which cells always have different behaviors compared with free non-encapsulated culture. In this work, the behaviors of K562 leukemia cells were studied once entrapped in solid and liquefied APA microcapsules as well as in free non-encapsulated culture. Glucose pulse culture was employed to characterize the growth and metabolism of microencapsulated K562 cells. And mathematical modeling was presented to develop a basis for the deeper understanding of cells responses to different culture environments. Based on the results of experiments and modeling, it was found that cells presented a better growing pattern and maintain the activity at a higher level for extending time. The concentration of lactate was higher in solid microcapsules culture than that of liquefied microcapsules culture, but the cell number was lower. And the lactate yield coefficients (lactate/glucose) were 0.8129, 0.6978 and 0.601 for free non-encapsulated, solid microcapsules and liquefied microcapsules culture, respectively. An increase of glucose concentration led a decrease of cell activity, The glucose consumption ratio were 99.9%, 86.8 %, 49.4% and 28.6% with the decrease in its concentration from 2 to 4, 6, 10 g/L, however, the lactate yield coefficient were 0.7184, 0.6654, 0.8239 and 0.9693, respectively. (c) 2006 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000240208800009
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/97155
专题中国科学院大连化学物理研究所
通讯作者Ma XJ(马小军); Ma XJ(马小军)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Sci & Technol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Ma, Juan,Qi, Wentao,Xie, Yubing,et al. K562 cell growth activity and metabolism characteristics in APA microencapsulated culture and modeling study[J]. JOURNAL OF BIOTECHNOLOGY,2006,125(2):242-251.
APA Ma, Juan.,Qi, Wentao.,Xie, Yubing.,Wang, Wei.,Yu, Weiting.,...&马小军.(2006).K562 cell growth activity and metabolism characteristics in APA microencapsulated culture and modeling study.JOURNAL OF BIOTECHNOLOGY,125(2),242-251.
MLA Ma, Juan,et al."K562 cell growth activity and metabolism characteristics in APA microencapsulated culture and modeling study".JOURNAL OF BIOTECHNOLOGY 125.2(2006):242-251.
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