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题名: K562 cell growth activity and metabolism characteristics in APA microencapsulated culture and modeling study
作者: Ma, Juan;  Qi, Wentao;  Xie, Yubing;  Wang, Wei;  Yu, Weiting;  Ma, Xiaojun
通讯作者: 马小军
关键词: microencapsulation ;  cell activity ;  cell growth and metabolism ;  modeling
刊名: JOURNAL OF BIOTECHNOLOGY
发表日期: 2006-09-01
DOI: 10.1016/j.jbiotec.2006.02.025
卷: 125, 期:2, 页:242-251
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Cell microencapsulation is likely to play a major role in cell and transplantation therapies in the next decade. The microcapsules provide a special microenvironment in which cells always have different behaviors compared with free non-encapsulated culture. In this work, the behaviors of K562 leukemia cells were studied once entrapped in solid and liquefied APA microcapsules as well as in free non-encapsulated culture. Glucose pulse culture was employed to characterize the growth and metabolism of microencapsulated K562 cells. And mathematical modeling was presented to develop a basis for the deeper understanding of cells responses to different culture environments. Based on the results of experiments and modeling, it was found that cells presented a better growing pattern and maintain the activity at a higher level for extending time. The concentration of lactate was higher in solid microcapsules culture than that of liquefied microcapsules culture, but the cell number was lower. And the lactate yield coefficients (lactate/glucose) were 0.8129, 0.6978 and 0.601 for free non-encapsulated, solid microcapsules and liquefied microcapsules culture, respectively. An increase of glucose concentration led a decrease of cell activity, The glucose consumption ratio were 99.9%, 86.8 %, 49.4% and 28.6% with the decrease in its concentration from 2 to 4, 6, 10 g/L, however, the lactate yield coefficient were 0.7184, 0.6654, 0.8239 and 0.9693, respectively. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: HUMAN FACTOR-IX ;  MAMMALIAN-CELLS ;  MICROCAPSULES ;  EXPRESSION ;  DELIVERY ;  LACTATE ;  ISLETS ;  KIDNEY ;  MICE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000240208800009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97155
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Sci & Technol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Ma, Juan,Qi, Wentao,Xie, Yubing,et al. K562 cell growth activity and metabolism characteristics in APA microencapsulated culture and modeling study[J]. JOURNAL OF BIOTECHNOLOGY,2006,125(2):242-251.
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