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K562 cell growth activity and metabolism characteristics in APA microencapsulated culture and modeling study
Ma, Juan; Qi, Wentao; Xie, Yubing; Wang, Wei; Yu, Weiting; Ma, Xiaojun; Ma XJ(马小军); Ma XJ(马小军)
KeywordMicroencapsulation Cell Activity Cell Growth And Metabolism Modeling
Source PublicationJOURNAL OF BIOTECHNOLOGY
2006-09-01
DOI10.1016/j.jbiotec.2006.02.025
Volume125Issue:2Pages:242-251
Indexed BySCI
SubtypeArticle
Department18
Funding Project1802
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology
WOS Research AreaBiotechnology & Applied Microbiology
WOS KeywordHUMAN FACTOR-IX ; MAMMALIAN-CELLS ; MICROCAPSULES ; EXPRESSION ; DELIVERY ; LACTATE ; ISLETS ; KIDNEY ; MICE
AbstractCell microencapsulation is likely to play a major role in cell and transplantation therapies in the next decade. The microcapsules provide a special microenvironment in which cells always have different behaviors compared with free non-encapsulated culture. In this work, the behaviors of K562 leukemia cells were studied once entrapped in solid and liquefied APA microcapsules as well as in free non-encapsulated culture. Glucose pulse culture was employed to characterize the growth and metabolism of microencapsulated K562 cells. And mathematical modeling was presented to develop a basis for the deeper understanding of cells responses to different culture environments. Based on the results of experiments and modeling, it was found that cells presented a better growing pattern and maintain the activity at a higher level for extending time. The concentration of lactate was higher in solid microcapsules culture than that of liquefied microcapsules culture, but the cell number was lower. And the lactate yield coefficients (lactate/glucose) were 0.8129, 0.6978 and 0.601 for free non-encapsulated, solid microcapsules and liquefied microcapsules culture, respectively. An increase of glucose concentration led a decrease of cell activity, The glucose consumption ratio were 99.9%, 86.8 %, 49.4% and 28.6% with the decrease in its concentration from 2 to 4, 6, 10 g/L, however, the lactate yield coefficient were 0.7184, 0.6654, 0.8239 and 0.9693, respectively. (c) 2006 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000240208800009
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/97155
Collection中国科学院大连化学物理研究所
Corresponding AuthorMa XJ(马小军); Ma XJ(马小军)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Sci & Technol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Ma, Juan,Qi, Wentao,Xie, Yubing,et al. K562 cell growth activity and metabolism characteristics in APA microencapsulated culture and modeling study[J]. JOURNAL OF BIOTECHNOLOGY,2006,125(2):242-251.
APA Ma, Juan.,Qi, Wentao.,Xie, Yubing.,Wang, Wei.,Yu, Weiting.,...&马小军.(2006).K562 cell growth activity and metabolism characteristics in APA microencapsulated culture and modeling study.JOURNAL OF BIOTECHNOLOGY,125(2),242-251.
MLA Ma, Juan,et al."K562 cell growth activity and metabolism characteristics in APA microencapsulated culture and modeling study".JOURNAL OF BIOTECHNOLOGY 125.2(2006):242-251.
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