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题名: CE-LIF coupled with flow cytometry for high-throughput quantitation of fluorophores in single intact cells
作者: Xiao, Hua;  Li, Xin;  Zou, Hanfa;  Yang, Ling;  Wang, Yulin;  Wang, Hailin;  Le, X. Chris
通讯作者: 邹汉法
关键词: apparent permeability coefficient ;  capillary electrophoresis ;  flow cytometry ;  single cell analysis ;  transport rate
刊名: ELECTROPHORESIS
发表日期: 2006-09-01
DOI: 10.1002/elps.200500536
卷: 27, 期:17, 页:3452-3459
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809,1806
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: We report a method of coupled CE-LIF detection with flow cytometry for high-throughput determination and quantitation of fluorophores in single intact K562/S (KS) cells. The membrane properties of KS cell including fluophore transport rate and apparent permeability coefficient were further quantitatively characterized. The method has advantages for accurate quantitation and unique capacity of high-throughput analysis. The strategy will be useful for the quantitation of fluorophores in the intact cells, such as measurement of multidrug resistance, quantitation of specific protein expression, and quantitative characterization of protein and enzyme functions.
关键词[WOS]: INDUCED FLUORESCENCE DETECTION ;  CAPILLARY-ELECTROPHORESIS ;  MULTIDRUG-RESISTANCE ;  CACO-2 MONOLAYERS ;  MAMMALIAN-CELLS ;  MICROSCOPY ;  TRANSPORT ;  EFFLUXES ;  PROTEIN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000240585900016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97219
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian, Peoples R China
3.Univ Alberta, Fac Med, Dept Publ Hlth Sci, Edmonton, AB, Canada

Recommended Citation:
Xiao, Hua,Li, Xin,Zou, Hanfa,et al. CE-LIF coupled with flow cytometry for high-throughput quantitation of fluorophores in single intact cells[J]. ELECTROPHORESIS,2006,27(17):3452-3459.
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