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Proliferation and differentiation of mouse embryonic stem cells in APA microcapsule: A model for studying the interaction between stem cells and their niche
Wang, XL; Wang, W; Ma, J; Guo, X; Yu, XJ; Ma, XJ; Ma XJ(马小军)
刊名BIOTECHNOLOGY PROGRESS
2006-06-01
DOI10.1021/bp050386n
22期:3页:791-800
收录类别SCI
文章类型Article
部门归属18
项目归属1802,1812
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology ; Food Science & Technology
研究领域[WOS]Biotechnology & Applied Microbiology ; Food Science & Technology
关键词[WOS]ALGINATE MICROCAPSULES ; SURVIVAL ; LINES
英文摘要Embryonic stem (ES) cells hold promise either as an in vitro model recapitulating early embryonic development or as a renewable source of therapeutically useful cells. Certain aspects of the microenvironment (or niche) play critical roles in determining the fate of ES cells. Here, we reported the feasibility of using the technique of microencapsulation to study the interaction between ES cells and their tissue niche. ES cells' growth, viability, and differentiation in vitro were evaluated when they were enclosed in solid or liquefied core APA microcapsules. In comparison with those microcapsules with solid cores, the liquefied capsules provided a more suitable culture environment for the growth of ES cells. In addition, behavior of encapsulated ES cells in vivo was observed after their being implanted into mouse peritoneal cavities. In contrast to the prolonged lag phase in vitro, ES cells encapsulated grew much faster in vivo. Typical markers for the undifferentiated ES cells, such as AP, SSEA-1, and Oct-4 gene, were also tracked by immunochemistry and RT-PCR. Results showed that expression of markers remained high over 2 weeks of culture in vitro. However, decreased expression of markers was found in those samples in vivo with time passage. These findings implied that it was the combination of the intrinsic characteristics of ES cells and their microenvironment that regulated their fate. The APA-ES cells system may provide an optimal model to study the interaction between stem cells and their tissue niches.
语种英语
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WOS记录号WOS:000237973500024
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被引频次:59[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/97227
专题中国科学院大连化学物理研究所
通讯作者Ma XJ(马小军)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China
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GB/T 7714
Wang, XL,Wang, W,Ma, J,et al. Proliferation and differentiation of mouse embryonic stem cells in APA microcapsule: A model for studying the interaction between stem cells and their niche[J]. BIOTECHNOLOGY PROGRESS,2006,22(3):791-800.
APA Wang, XL.,Wang, W.,Ma, J.,Guo, X.,Yu, XJ.,...&马小军.(2006).Proliferation and differentiation of mouse embryonic stem cells in APA microcapsule: A model for studying the interaction between stem cells and their niche.BIOTECHNOLOGY PROGRESS,22(3),791-800.
MLA Wang, XL,et al."Proliferation and differentiation of mouse embryonic stem cells in APA microcapsule: A model for studying the interaction between stem cells and their niche".BIOTECHNOLOGY PROGRESS 22.3(2006):791-800.
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