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Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptides and MALDI-TOF MS analysis
Zhou, Houjiang; Xu, Songyun; Ye, Mingliang; Feng, Shun; Pan, Chensong; Jiang, Xiaogang; Li, Xin; Han, Guanghui; Fu, Yu; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Zirconium Phosphonate Porous Silicon Phosphopeptide Maldi-tof Ms
刊名JOURNAL OF PROTEOME RESEARCH
2006-09-01
ISSN1535-3893
DOI10.1021/pr060162f
5期:9页:2431-2437
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]DESORPTION/IONIZATION-MASS-SPECTROMETRY ; ION AFFINITY-CHROMATOGRAPHY ; PHOSPHORYLATED PEPTIDES ; PROTEIN-PHOSPHORYLATION ; PHOSPHOPROTEOME ANALYSIS ; SELECTIVE ENRICHMENT ; IDENTIFICATION ; PLATFORM ; MS/MS ; NANOPARTICLES
英文摘要Phosphorylation is one of the most important post-translational modifications of proteins, which modulates a wide range of biological functions and activity of proteins. The analysis of phosphopeptides is still one of the most challenging tasks in proteomics research by mass spectrometry. In this study, a novel phosphopeptide enrichment approach based on the strong interaction of zirconium phosphonate (ZrP) modified surface with phosphopeptides has been developed. ZrP modified porous silicon (ZrPpSi) wafer was prepared to specifically capture the phosphopeptides from complex peptide mixtures, and then the captured phosphopeptides were analyzed by MALDI-TOF MS by directly placing the wafer on a MALDI target. The phosphopeptide enrichment and MALDI analysis were both performed on the ZrP-pSi wafer which significantly reduced the sample loss and simplified the analytical procedures. The prepared ZrP-pSi wafer has been successfully applied for the enrichment of phosphopeptides from the tryptic digest of standard phosphoproteins, beta-casein and alpha-casein. The excellent selectivity of this approach was demonstrated by analyzing phosphopeptides in the digest mixture of beta-casein and bovine serum albumin with molar ratio of 1:100. High detection sensitivity has been achieved for the analysis of the phosphopeptides from tryptic digestion of 2 fmol, beta-casein on the ZrP-pSi surface.
语种英语
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WOS记录号WOS:000240200700041
引用统计
被引频次:135[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/97235
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Zhou, Houjiang,Xu, Songyun,Ye, Mingliang,et al. Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptides and MALDI-TOF MS analysis[J]. JOURNAL OF PROTEOME RESEARCH,2006,5(9):2431-2437.
APA Zhou, Houjiang.,Xu, Songyun.,Ye, Mingliang.,Feng, Shun.,Pan, Chensong.,...&邹汉法.(2006).Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptides and MALDI-TOF MS analysis.JOURNAL OF PROTEOME RESEARCH,5(9),2431-2437.
MLA Zhou, Houjiang,et al."Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptides and MALDI-TOF MS analysis".JOURNAL OF PROTEOME RESEARCH 5.9(2006):2431-2437.
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