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题名: Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptides and MALDI-TOF MS analysis
作者: Zhou, Houjiang;  Xu, Songyun;  Ye, Mingliang;  Feng, Shun;  Pan, Chensong;  Jiang, Xiaogang;  Li, Xin;  Han, Guanghui;  Fu, Yu;  Zou, Hanfa
通讯作者: 邹汉法
关键词: zirconium phosphonate ;  porous silicon ;  phosphopeptide ;  MALDI-TOF MS
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2006-09-01
DOI: 10.1021/pr060162f
卷: 5, 期:9, 页:2431-2437
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Phosphorylation is one of the most important post-translational modifications of proteins, which modulates a wide range of biological functions and activity of proteins. The analysis of phosphopeptides is still one of the most challenging tasks in proteomics research by mass spectrometry. In this study, a novel phosphopeptide enrichment approach based on the strong interaction of zirconium phosphonate (ZrP) modified surface with phosphopeptides has been developed. ZrP modified porous silicon (ZrPpSi) wafer was prepared to specifically capture the phosphopeptides from complex peptide mixtures, and then the captured phosphopeptides were analyzed by MALDI-TOF MS by directly placing the wafer on a MALDI target. The phosphopeptide enrichment and MALDI analysis were both performed on the ZrP-pSi wafer which significantly reduced the sample loss and simplified the analytical procedures. The prepared ZrP-pSi wafer has been successfully applied for the enrichment of phosphopeptides from the tryptic digest of standard phosphoproteins, beta-casein and alpha-casein. The excellent selectivity of this approach was demonstrated by analyzing phosphopeptides in the digest mixture of beta-casein and bovine serum albumin with molar ratio of 1:100. High detection sensitivity has been achieved for the analysis of the phosphopeptides from tryptic digestion of 2 fmol, beta-casein on the ZrP-pSi surface.
关键词[WOS]: DESORPTION/IONIZATION-MASS-SPECTROMETRY ;  ION AFFINITY-CHROMATOGRAPHY ;  PHOSPHORYLATED PEPTIDES ;  PROTEIN-PHOSPHORYLATION ;  PHOSPHOPROTEOME ANALYSIS ;  SELECTIVE ENRICHMENT ;  IDENTIFICATION ;  PLATFORM ;  MS/MS ;  NANOPARTICLES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000240200700041
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97235
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Zhou, Houjiang,Xu, Songyun,Ye, Mingliang,et al. Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptides and MALDI-TOF MS analysis[J]. JOURNAL OF PROTEOME RESEARCH,2006,5(9):2431-2437.
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