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Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS
Kong, Liang; Yu, Zhiyuan; Bao, Yongming; Su, Xingye; Zou, Hanfa; Li, Xin; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Coniferyl Ferulate Ferulic Acid Ethyl Ester Hplc-ms Rhizoma Chuanxiong Traditional Chinese Medicine
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2006-09-01
ISSN1618-2642
DOI10.1007/s00216-006-0621-0
386期:2页:264-274
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; FERULIC ACID ; CNIDIUM-OFFICINALE ; ANGELICA-SINENSIS ; TETRAMETHYLPYRAZINE ; HYDROXYPHTHALIDES ; PREGNENOLONE ; INGREDIENTS ; COMPONENTS
英文摘要A new screening and analysis method that combines in vitro metabolism with high-performance liquid chromatography-mass spectrometry (HPLC-MS) was developed for the screening and analysis of an antineoplastic compound, coniferyl ferulate, which is present in the rhizome of Rhizoma Chuanxiong. Infrared (IR), ultraviolet visible spectroscopy (UV-Vis), nuclear magnetic resonance (NMR) and element analysis were used to identify the molecular structure of coniferyl ferulate. The quantitative analysis of coniferyl ferulate in different extracts of Rhizoma Chuanxiong was carried out, and the metabolism of coniferyl ferulate was investigated by in vitro incubation with rat liver homogenate. The metabolite of coniferyl ferulate, ferulic acid ethyl ester, was identified by HPLC-MS, UV-Vis and IR. In addition, antineoplastic activities of coniferyl ferulate and ferulic acid ethyl ester were detected by the MTT assay. The observed inhibition rate of coniferyl ferulate on the activity of HeLa cells was over 80% at 5.4 ng mu l(-1). However, its metabolite, ferulic acid ethyl ester, showed no antineoplastic activity in vitro.
语种英语
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WOS记录号WOS:000240281400010
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/97285
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian 116024, Peoples R China
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GB/T 7714
Kong, Liang,Yu, Zhiyuan,Bao, Yongming,et al. Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,386(2):264-274.
APA Kong, Liang.,Yu, Zhiyuan.,Bao, Yongming.,Su, Xingye.,Zou, Hanfa.,...&邹汉法.(2006).Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,386(2),264-274.
MLA Kong, Liang,et al."Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 386.2(2006):264-274.
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