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题名: Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS
作者: Kong, Liang;  Yu, Zhiyuan;  Bao, Yongming;  Su, Xingye;  Zou, Hanfa;  Li, Xin
通讯作者: 邹汉法
关键词: coniferyl ferulate ;  ferulic acid ethyl ester ;  HPLC-MS ;  rhizoma chuanxiong ;  traditional Chinese medicine
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2006-09-01
DOI: 10.1007/s00216-006-0621-0
卷: 386, 期:2, 页:264-274
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A new screening and analysis method that combines in vitro metabolism with high-performance liquid chromatography-mass spectrometry (HPLC-MS) was developed for the screening and analysis of an antineoplastic compound, coniferyl ferulate, which is present in the rhizome of Rhizoma Chuanxiong. Infrared (IR), ultraviolet visible spectroscopy (UV-Vis), nuclear magnetic resonance (NMR) and element analysis were used to identify the molecular structure of coniferyl ferulate. The quantitative analysis of coniferyl ferulate in different extracts of Rhizoma Chuanxiong was carried out, and the metabolism of coniferyl ferulate was investigated by in vitro incubation with rat liver homogenate. The metabolite of coniferyl ferulate, ferulic acid ethyl ester, was identified by HPLC-MS, UV-Vis and IR. In addition, antineoplastic activities of coniferyl ferulate and ferulic acid ethyl ester were detected by the MTT assay. The observed inhibition rate of coniferyl ferulate on the activity of HeLa cells was over 80% at 5.4 ng mu l(-1). However, its metabolite, ferulic acid ethyl ester, showed no antineoplastic activity in vitro.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  FERULIC ACID ;  CNIDIUM-OFFICINALE ;  ANGELICA-SINENSIS ;  TETRAMETHYLPYRAZINE ;  HYDROXYPHTHALIDES ;  PREGNENOLONE ;  INGREDIENTS ;  COMPONENTS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000240281400010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97285
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian 116024, Peoples R China

Recommended Citation:
Kong, Liang,Yu, Zhiyuan,Bao, Yongming,et al. Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,386(2):264-274.
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