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题名: Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach
作者: Tian, Ruijun;  Jiang, Xinning;  Li, Xin;  Jiang, Xiaogang;  Feng, Shun;  Xu, Songyun;  Han, Guanghui;  Ye, Mingliang;  Zou, Hanfa
通讯作者: 邹汉法
关键词: chemiproteomics ;  library screening ;  interacting proteins ;  p-aminobenzamidine ;  trypsin-like serine proteases ;  human plasma
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2006-11-17
DOI: 10.1016/j.chroma.2006.08.097
卷: 1134, 期:1-2, 页:134-142
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this study, a gel free chemiproteomic method based on chromatography was developed and applied for the biological fingerprinting analysis of complex biological system. p-Aminobenzamidine (ABA), an inhibitor of trypsin-like serine proteases, was immobilized for characterizing their interacting proteins in human plasma. By the proteomic analysis method, 214 proteins were identified with obvious affinity to the immobilized ABA. By searching the sequences of above proteins with consensus patterns of the two active sites, seven proteins belong to trypsin-like serine protease group were found. Based on the Gene Ontology annotation, the identified trypsin-like serine proteases have the function of catalytic activity and calcium ion binding, and are mainly involved in the biological process of blood coagulation. Eight more other proteins related to calcium ion binding and blood coagulation were found. Nearly all of these proteins cannot be identified by directly analyzing the plasma sample demonstrating the chemiproteomics a useful approach to characterize interacting proteins in the low abundance range. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE AFFINITY-CHROMATOGRAPHY ;  PROTEIN INTERACTIONS ;  CHEMICAL PROTEOMICS ;  PURIFICATION ;  BINDING ;  IDENTIFICATION ;  STRATEGIES ;  SYSTEM ;  TARGET
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000242059400017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97437
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Tian, Ruijun,Jiang, Xinning,Li, Xin,et al. Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1134(1-2):134-142.
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