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Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach
Tian, Ruijun; Jiang, Xinning; Li, Xin; Jiang, Xiaogang; Feng, Shun; Xu, Songyun; Han, Guanghui; Ye, Mingliang; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Chemiproteomics Library Screening Interacting Proteins P-aminobenzamidine Trypsin-like Serine Proteases Human Plasma
刊名JOURNAL OF CHROMATOGRAPHY A
2006-11-17
ISSN0021-9673
DOI10.1016/j.chroma.2006.08.097
1134期:1-2页:134-142
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE AFFINITY-CHROMATOGRAPHY ; PROTEIN INTERACTIONS ; CHEMICAL PROTEOMICS ; PURIFICATION ; BINDING ; IDENTIFICATION ; STRATEGIES ; SYSTEM ; TARGET
英文摘要In this study, a gel free chemiproteomic method based on chromatography was developed and applied for the biological fingerprinting analysis of complex biological system. p-Aminobenzamidine (ABA), an inhibitor of trypsin-like serine proteases, was immobilized for characterizing their interacting proteins in human plasma. By the proteomic analysis method, 214 proteins were identified with obvious affinity to the immobilized ABA. By searching the sequences of above proteins with consensus patterns of the two active sites, seven proteins belong to trypsin-like serine protease group were found. Based on the Gene Ontology annotation, the identified trypsin-like serine proteases have the function of catalytic activity and calcium ion binding, and are mainly involved in the biological process of blood coagulation. Eight more other proteins related to calcium ion binding and blood coagulation were found. Nearly all of these proteins cannot be identified by directly analyzing the plasma sample demonstrating the chemiproteomics a useful approach to characterize interacting proteins in the low abundance range. (c) 2006 Elsevier B.V. All rights reserved.
语种英语
原文出处查看原文
WOS记录号WOS:000242059400017
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/97437
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Tian, Ruijun,Jiang, Xinning,Li, Xin,et al. Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1134(1-2):134-142.
APA Tian, Ruijun.,Jiang, Xinning.,Li, Xin.,Jiang, Xiaogang.,Feng, Shun.,...&邹汉法.(2006).Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach.JOURNAL OF CHROMATOGRAPHY A,1134(1-2),134-142.
MLA Tian, Ruijun,et al."Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach".JOURNAL OF CHROMATOGRAPHY A 1134.1-2(2006):134-142.
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