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Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach
Tian, Ruijun; Jiang, Xinning; Li, Xin; Jiang, Xiaogang; Feng, Shun; Xu, Songyun; Han, Guanghui; Ye, Mingliang; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
KeywordChemiproteomics Library Screening Interacting Proteins P-aminobenzamidine Trypsin-like Serine Proteases Human Plasma
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2006-11-17
ISSN0021-9673
DOI10.1016/j.chroma.2006.08.097
Volume1134Issue:1-2Pages:134-142
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE AFFINITY-CHROMATOGRAPHY ; PROTEIN INTERACTIONS ; CHEMICAL PROTEOMICS ; PURIFICATION ; BINDING ; IDENTIFICATION ; STRATEGIES ; SYSTEM ; TARGET
AbstractIn this study, a gel free chemiproteomic method based on chromatography was developed and applied for the biological fingerprinting analysis of complex biological system. p-Aminobenzamidine (ABA), an inhibitor of trypsin-like serine proteases, was immobilized for characterizing their interacting proteins in human plasma. By the proteomic analysis method, 214 proteins were identified with obvious affinity to the immobilized ABA. By searching the sequences of above proteins with consensus patterns of the two active sites, seven proteins belong to trypsin-like serine protease group were found. Based on the Gene Ontology annotation, the identified trypsin-like serine proteases have the function of catalytic activity and calcium ion binding, and are mainly involved in the biological process of blood coagulation. Eight more other proteins related to calcium ion binding and blood coagulation were found. Nearly all of these proteins cannot be identified by directly analyzing the plasma sample demonstrating the chemiproteomics a useful approach to characterize interacting proteins in the low abundance range. (c) 2006 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000242059400017
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Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/97437
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Tian, Ruijun,Jiang, Xinning,Li, Xin,et al. Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1134(1-2):134-142.
APA Tian, Ruijun.,Jiang, Xinning.,Li, Xin.,Jiang, Xiaogang.,Feng, Shun.,...&邹汉法.(2006).Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach.JOURNAL OF CHROMATOGRAPHY A,1134(1-2),134-142.
MLA Tian, Ruijun,et al."Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach".JOURNAL OF CHROMATOGRAPHY A 1134.1-2(2006):134-142.
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