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题名: Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes
作者: Hu, Ligang;  Jiang, Guibin;  Xu, Songyun;  Pan, Chensong;  Zou, Hanfa
通讯作者: 邹汉法
刊名: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
发表日期: 2006-11-01
DOI: 10.1016/j.jasms.2006.07.005
卷: 17, 期:11, 页:1616-1619
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 2;3
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Analytical ;  Chemistry, Physical ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Spectroscopy
英文摘要: A matrix assisted laser desorption/ionization time-of-flight mass spectrometry platform for quantitatively monitoring enzyme activity and screening enzyme inhibitors has been demonstrated. The described method employs a new matrix of oxidized carbon nanotubes. Compared with the traditional fluorescence approach, this label-free method has the advantage of directly identifying the substrates and products in enzymatic reactions. Moreover, the method could be conveniently carried out with any commercial mass spectrometer without modification. We quantitatively monitored the acetylcholinesterase activity and screened acetylcholinesterase inhibitors with a detection rate of about 3.3 s per sample.
关键词[WOS]: FLIGHT MASS-SPECTROMETRY ;  DESORPTION/IONIZATION ;  SYSTEM
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000242242900017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97443
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China

Recommended Citation:
Hu, Ligang,Jiang, Guibin,Xu, Songyun,et al. Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2006,17(11):1616-1619.
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