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Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes
Hu, Ligang; Jiang, Guibin; Xu, Songyun; Pan, Chensong; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
刊名JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
2006-11-01
ISSN1044-0305
DOI10.1016/j.jasms.2006.07.005
17期:11页:1616-1619
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名2;3
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Analytical ; Chemistry, Physical ; Spectroscopy
研究领域[WOS]Chemistry ; Spectroscopy
关键词[WOS]FLIGHT MASS-SPECTROMETRY ; DESORPTION/IONIZATION ; SYSTEM
英文摘要A matrix assisted laser desorption/ionization time-of-flight mass spectrometry platform for quantitatively monitoring enzyme activity and screening enzyme inhibitors has been demonstrated. The described method employs a new matrix of oxidized carbon nanotubes. Compared with the traditional fluorescence approach, this label-free method has the advantage of directly identifying the substrates and products in enzymatic reactions. Moreover, the method could be conveniently carried out with any commercial mass spectrometer without modification. We quantitatively monitored the acetylcholinesterase activity and screened acetylcholinesterase inhibitors with a detection rate of about 3.3 s per sample.
语种英语
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WOS记录号WOS:000242242900017
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被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/97443
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
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GB/T 7714
Hu, Ligang,Jiang, Guibin,Xu, Songyun,et al. Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2006,17(11):1616-1619.
APA Hu, Ligang.,Jiang, Guibin.,Xu, Songyun.,Pan, Chensong.,Zou, Hanfa.,...&邹汉法.(2006).Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,17(11),1616-1619.
MLA Hu, Ligang,et al."Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 17.11(2006):1616-1619.
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