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题名: Simultaneous analysis of microsatellite instability and loss of heterozygosity by capillary electrophoresis with a homemade kit
作者: Shi, XZ;  Li, JH;  Li, AY;  Lv, S;  Xu, GW
通讯作者: 许国旺
关键词: microsatellite instability ;  loss of heterozygosity ;  capillary electrophoresis
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2006-04-13
DOI: 10.1016/j.jchromb.2006.02.052
卷: 834, 期:1-2, 页:122-127
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Microsatellite instability (MSI) and loss of heterozygosity (LOH), the alteration in length and strength of short tandem repeat sequences are an important molecular characteristic of many human tumors. MSI and LOH analysis has become an attractive method for diagnostic and tumor research purposes. A method for the simultaneous analysis of MSI and LOH at the five microsatellite loci (BAT-26, D17S261, D3S1283, D2S123 and D3S133S D3S1611) was developed employing a cheap homemade kit to replace the expensive cornmercial kit on ABI 310 capillary genetic analyzer. After studying the effect of temperature and urea denaturant on microsatellite analysis, 8 mol/L urea and 60 degrees C were selected for assessing accurately fragment size of microsatellite alleles. Based on this method, 52 sporadic gastric cancers were screened, and MSI and LOH, at least one locus was observed in 15 of 52 (28.8%) patients. Moreover, it is found that a statistically significant association exists between MSI and LOH and tumor-differentiated level. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: STRAND CONFORMATION POLYMORPHISM ;  LASER-INDUCED FLUORESCENCE ;  POLYMERASE-CHAIN-REACTION ;  TANDEM REPEAT LOCI ;  K-RAS GENE ;  REPEATED SEQUENCES ;  X-CHROMOSOME ;  MUTATION ;  DNA ;  POPULATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000236927600016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97603
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China
3.Haebin Med Univ, Hosp Affiliated, Haerbin 150001, Peoples R China

Recommended Citation:
Shi, XZ,Li, JH,Li, AY,et al. Simultaneous analysis of microsatellite instability and loss of heterozygosity by capillary electrophoresis with a homemade kit[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,834(1-2):122-127.
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