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Simultaneous analysis of microsatellite instability and loss of heterozygosity by capillary electrophoresis with a homemade kit
Shi, XZ; Li, JH; Li, AY; Lv, S; Xu, GW; Xu GW(许国旺); Xu GW(许国旺)
KeywordMicrosatellite Instability Loss Of Heterozygosity Capillary Electrophoresis
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2006-04-13
DOI10.1016/j.jchromb.2006.02.052
Volume834Issue:1-2Pages:122-127
Indexed BySCI
SubtypeArticle
Department18
Funding Project1808
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordSTRAND CONFORMATION POLYMORPHISM ; LASER-INDUCED FLUORESCENCE ; POLYMERASE-CHAIN-REACTION ; TANDEM REPEAT LOCI ; K-RAS GENE ; REPEATED SEQUENCES ; X-CHROMOSOME ; MUTATION ; DNA ; POPULATION
AbstractMicrosatellite instability (MSI) and loss of heterozygosity (LOH), the alteration in length and strength of short tandem repeat sequences are an important molecular characteristic of many human tumors. MSI and LOH analysis has become an attractive method for diagnostic and tumor research purposes. A method for the simultaneous analysis of MSI and LOH at the five microsatellite loci (BAT-26, D17S261, D3S1283, D2S123 and D3S133S D3S1611) was developed employing a cheap homemade kit to replace the expensive cornmercial kit on ABI 310 capillary genetic analyzer. After studying the effect of temperature and urea denaturant on microsatellite analysis, 8 mol/L urea and 60 degrees C were selected for assessing accurately fragment size of microsatellite alleles. Based on this method, 52 sporadic gastric cancers were screened, and MSI and LOH, at least one locus was observed in 15 of 52 (28.8%) patients. Moreover, it is found that a statistically significant association exists between MSI and LOH and tumor-differentiated level. (c) 2006 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000236927600016
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/97603
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺); Xu GW(许国旺)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China
3.Haebin Med Univ, Hosp Affiliated, Haerbin 150001, Peoples R China
Recommended Citation
GB/T 7714
Shi, XZ,Li, JH,Li, AY,et al. Simultaneous analysis of microsatellite instability and loss of heterozygosity by capillary electrophoresis with a homemade kit[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,834(1-2):122-127.
APA Shi, XZ.,Li, JH.,Li, AY.,Lv, S.,Xu, GW.,...&许国旺.(2006).Simultaneous analysis of microsatellite instability and loss of heterozygosity by capillary electrophoresis with a homemade kit.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,834(1-2),122-127.
MLA Shi, XZ,et al."Simultaneous analysis of microsatellite instability and loss of heterozygosity by capillary electrophoresis with a homemade kit".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 834.1-2(2006):122-127.
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