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题名: Rapid detection of Staphylococcus aureus by a combination of monoclonal antibody-coated latex and capillary electrophoresis
作者: Gao, P;  Xu, GW;  Shi, XZ;  Yuan, KL;  Tian, J
通讯作者: 许国旺
关键词: bacterial analysis ;  capillary electrophoresis ;  monoclonal antibody ;  pathogenic bacteria ;  Staphylococcus aureus
刊名: ELECTROPHORESIS
发表日期: 2006-05-01
DOI: 10.1002/elps.200500656
卷: 27, 期:9, 页:1784-1789
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The rapid detection of pathogenic bacteria is extremely important in biotechnology and clinical diagnosis. CE has been utilized in the field of bacterial analysis for many years, but to some extent, simultaneous separation and identification of certain microbes from complex samples by CE coupled with UV detector is still a challenge. In this paper, we propose a new strategy for rapid separation and identification of Staphylococcus aureus (S. aureus) in bacterial mixtures by means of specific mAb-coated latex coupled with CZE. An appropriate set of conditions that selectively isolated S. aureus from the microorganisms Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae were established. S. aureus could be differentiated from the others by unique peaks in the electropherograms. The validity was also confirmed by LIF with antibodies specific to both the latex and the microbial cells. The LOD is as low as 9.0 x 10(5) colony forming unit/mL. We have also utilized this technology to identify S. aureus in a stool sample coming from a healthy volunteer spiked successfully with S. aureus. This CZE-UV technique can be applied to rapid diagnosis of enteritis caused by S. aureus or other bacterial control-related fields needing rapid identification of target pathogens from microbial mixtures. In theory, this method is suitable for the detection of any bacterium as long as corresponding bacterium-specific antibody-coated latex is available.
关键词[WOS]: LASER-INDUCED FLUORESCENCE ;  HIGH-PERFORMANCE ELECTROPHORESIS ;  ZONE-ELECTROPHORESIS ;  PATHOGENIC BACTERIA ;  SENSITIVE DETECTION ;  SEPARATION ;  CE ;  IDENTIFICATION ;  NANOPARTICLES ;  INFECTIONS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000237685600013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97605
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Gao, P,Xu, GW,Shi, XZ,et al. Rapid detection of Staphylococcus aureus by a combination of monoclonal antibody-coated latex and capillary electrophoresis[J]. ELECTROPHORESIS,2006,27(9):1784-1789.
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