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题名: Metabonomics study of intestinal fistulas based on ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry (UPLC/Q-TOF MS)
作者: Yin, Peiyuan;  Zhao, Xinjie;  Li, Qiurong;  Wang, Jiangshan;  Li, Jieshou;  Xu, Guowang
通讯作者: 许国旺
关键词: metabonomics ;  intestinal fistula ;  UPLC/Q-TOF MS ;  biomarker
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2006-09-01
DOI: 10.1021/pr060256p
卷: 5, 期:9, 页:2135-2143
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry (UPLC/Q-TOF MS) is an effective and sensitive analytical tool. A UPLC/Q-TOF MS-based metabonomics technique was employed to investigate sera from 40 patients with intestinal fistula and 17 healthy volunteers in an effort to find potential biomarkers of the disease and reveal their pathophysiological changes. After the UPLC/Q-TOF analysis, the retention time and m/z data pair for each peak were detected. Partial least squares discriminant analysis (PLS-DA) and coefficient of correlation analysis were used for marker selection and identification. According to the data, nine potential biomarkers were identified: glycochenodeoxycholic acid, glycodeoxycholic acid, taurochenodexycholic acid, taurodeoxycholic acid, and two kinds of lysophosphatidyl choline (C16: 0 and C18:2) were found with increased concentrations in the patients, and phenylalanine, tryptophan, and carnitine were found with decreased concentrations in the patients. The results suggested that a subclinical hepatic injury and abnormal metabolism of two essential amino acids ( phenylalanine and tryptophan), and a key compound of fatty acid synthesis and beta-oxidation (carnitine), occurred in the fistula patients. This work demonstrates the utility of metabonomics as a top-down systems biology tool for understanding clinical problems.
关键词[WOS]: BIOLOGICAL-FLUIDS ;  BILE-ACIDS ;  METABOLISM ;  SYSTEMS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000240200700009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97615
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Gen Hosp, Nanjing Command PLA, Nanjing 210002, Peoples R China

Recommended Citation:
Yin, Peiyuan,Zhao, Xinjie,Li, Qiurong,et al. Metabonomics study of intestinal fistulas based on ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry (UPLC/Q-TOF MS)[J]. JOURNAL OF PROTEOME RESEARCH,2006,5(9):2135-2143.
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