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High performance liquid chromatography-mass spectrometry for metabonomics: Potential biomarkers for acute deterioration of liver function in chronic hepatitis B
Yang, J; Zhao, XJ; Liu, XL; Wang, C; Gao, P; Wang, JS; Li, LJ; Gu, JR; Yang, SL; Xu, GW; Xu GW(许国旺); Xu GW(许国旺)
KeywordMetabonomics Hepatitis Lc Ms Pls-da Serum Biomarker
Source PublicationJOURNAL OF PROTEOME RESEARCH
2006-03-01
DOI10.1021/pr050364w
Volume5Issue:3Pages:554-561
Indexed BySCI
SubtypeArticle
Department18
Funding Project1808
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordMULTIVARIATE STATISTICAL-ANALYSIS ; HIGH-RESOLUTION NMR ; H-1-NMR SPECTROSCOPY ; PATTERN-RECOGNITION ; IDENTIFYING DIFFERENCES ; METABOLOMIC ANALYSES ; HIGH-THROUGHPUT ; OA-TOF ; SAMPLES ; URINE
AbstractMetabonomics methods have been successfully applied to the drug discovery, toxicology, phytochemistry, and clinical fields. Here, we report a self-developed metabonomics platform which is based on high performance liquid chromatog raphy- mass spectrometry (HPLC-MS) technique and applied to the investigation of acute deterioration of liver function in chronic hepatitis B to find the potential biomarkers. Sera from 50 healthy persons and 37 patients with acute deterioration of liver function in chronic hepatitis B were analyzed by HPLC-MS after removal of proteins. After de-noise, peak detection A, and peak alignment, the data of metabolites were fed to partial least squares discriminant analysis (PLS-DA) to find the potential biomarkers. According to the corresponding tandem mass results, several potential biomarkers were identified: Lysophosphaticlyl Choline (LPC) C18:0, LPC C16:0, LPC C18:1, LPC C18:2, and glycochenodeoxycholic acid (GCDCA) (or its isomer glycocleoxycholic acid (GDCA)). On the basis of the relevant literature and pathway databases, the biological significance of the present study is discussed.
Language英语
URL查看原文
WOS IDWOS:000235897000011
Citation statistics
Cited Times:113[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/97617
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺); Xu GW(许国旺)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Zhejiang Univ, Affiliated Hosp 1, Coll Med, Hangzhou 310027, Peoples R China
3.Shanghai Jiao Tong Univ, Shanghai Canc Inst, Natl Lab Oncogenes & Related Genes, Shanghai 200032, Peoples R China
4.Chinese Acad Sci, Shanghai Res Ctr Biotechnol, Shanghai, Peoples R China
Recommended Citation
GB/T 7714
Yang, J,Zhao, XJ,Liu, XL,et al. High performance liquid chromatography-mass spectrometry for metabonomics: Potential biomarkers for acute deterioration of liver function in chronic hepatitis B[J]. JOURNAL OF PROTEOME RESEARCH,2006,5(3):554-561.
APA Yang, J.,Zhao, XJ.,Liu, XL.,Wang, C.,Gao, P.,...&许国旺.(2006).High performance liquid chromatography-mass spectrometry for metabonomics: Potential biomarkers for acute deterioration of liver function in chronic hepatitis B.JOURNAL OF PROTEOME RESEARCH,5(3),554-561.
MLA Yang, J,et al."High performance liquid chromatography-mass spectrometry for metabonomics: Potential biomarkers for acute deterioration of liver function in chronic hepatitis B".JOURNAL OF PROTEOME RESEARCH 5.3(2006):554-561.
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