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题名: High performance liquid chromatography-mass spectrometry for metabonomics: Potential biomarkers for acute deterioration of liver function in chronic hepatitis B
作者: Yang, J;  Zhao, XJ;  Liu, XL;  Wang, C;  Gao, P;  Wang, JS;  Li, LJ;  Gu, JR;  Yang, SL;  Xu, GW
通讯作者: 许国旺
关键词: metabonomics ;  hepatitis ;  LC ;  MS ;  PLS-DA ;  serum ;  biomarker
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2006-03-01
DOI: 10.1021/pr050364w
卷: 5, 期:3, 页:554-561
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Metabonomics methods have been successfully applied to the drug discovery, toxicology, phytochemistry, and clinical fields. Here, we report a self-developed metabonomics platform which is based on high performance liquid chromatog raphy- mass spectrometry (HPLC-MS) technique and applied to the investigation of acute deterioration of liver function in chronic hepatitis B to find the potential biomarkers. Sera from 50 healthy persons and 37 patients with acute deterioration of liver function in chronic hepatitis B were analyzed by HPLC-MS after removal of proteins. After de-noise, peak detection A, and peak alignment, the data of metabolites were fed to partial least squares discriminant analysis (PLS-DA) to find the potential biomarkers. According to the corresponding tandem mass results, several potential biomarkers were identified: Lysophosphaticlyl Choline (LPC) C18:0, LPC C16:0, LPC C18:1, LPC C18:2, and glycochenodeoxycholic acid (GCDCA) (or its isomer glycocleoxycholic acid (GDCA)). On the basis of the relevant literature and pathway databases, the biological significance of the present study is discussed.
关键词[WOS]: MULTIVARIATE STATISTICAL-ANALYSIS ;  HIGH-RESOLUTION NMR ;  H-1-NMR SPECTROSCOPY ;  PATTERN-RECOGNITION ;  IDENTIFYING DIFFERENCES ;  METABOLOMIC ANALYSES ;  HIGH-THROUGHPUT ;  OA-TOF ;  SAMPLES ;  URINE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000235897000011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97617
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Zhejiang Univ, Affiliated Hosp 1, Coll Med, Hangzhou 310027, Peoples R China
3.Shanghai Jiao Tong Univ, Shanghai Canc Inst, Natl Lab Oncogenes & Related Genes, Shanghai 200032, Peoples R China
4.Chinese Acad Sci, Shanghai Res Ctr Biotechnol, Shanghai, Peoples R China

Recommended Citation:
Yang, J,Zhao, XJ,Liu, XL,et al. High performance liquid chromatography-mass spectrometry for metabonomics: Potential biomarkers for acute deterioration of liver function in chronic hepatitis B[J]. JOURNAL OF PROTEOME RESEARCH,2006,5(3):554-561.
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