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题名: Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection
作者: Pang, T;  Bai, CM;  Xu, YJ;  Xu, GW;  Yuan, ZY;  Su, Y;  Peng, LM
通讯作者: 许国旺
关键词: solid-phase extraction ;  liquid chromatography ;  evaporative light scattering ;  tobacco leaf ;  sugars
刊名: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
发表日期: 2006
DOI: 10.1080/10826070600598993
卷: 29, 期:9, 页:1281-1289
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A reversed phase liquid chromatography-evaporative light scattering detection, combined with solid phase extraction method was developed for the separation and determination of sugars in tobacco leaf. Separation was performed with a Hypersil NH2 analytical column and the mobile phase 75%:25% acetonitrile-water. In the range of 0.1-4 mg/mL, the method showed good linearity, with the correlation coefficients exceeding 0.99. The limits of detection of xylose, fructose, glucose, sucrose, maltose, and raffinose were 2, 2, 3, 2, 4, and 4 mu g/mL, respectively. The relative standard deviations of peak areas for these sugars were in the range of 2-3%. Recoveries of fructose, glucose, and sucrose were between 96 and 117%. Finally, determination of sugars in tobacco leaf, before and after bio-catalysis, was performed with this method.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ORGANIC-ACIDS ;  GAS-CHROMATOGRAPHY ;  EXTRACTS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000237497300006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97621
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Biochem & Cell Biol Inst, Shanghai Inst Biol Sci, Shanghai, Peoples R China
3.Chuxiong Cigarette Co, Hongta Grp, Yunnan, Peoples R China

Recommended Citation:
Pang, T,Bai, CM,Xu, YJ,et al. Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2006,29(9):1281-1289.
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