DICP OpenIR
Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection
Pang, T; Bai, CM; Xu, YJ; Xu, GW; Yuan, ZY; Su, Y; Peng, LM; Xu GW(许国旺); Xu GW(许国旺)
KeywordSolid-phase Extraction Liquid Chromatography Evaporative Light Scattering Tobacco Leaf Sugars
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
2006
DOI10.1080/10826070600598993
Volume29Issue:9Pages:1281-1289
Indexed BySCI
SubtypeArticle
Department18
Funding Project1808
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; ORGANIC-ACIDS ; GAS-CHROMATOGRAPHY ; EXTRACTS
AbstractA reversed phase liquid chromatography-evaporative light scattering detection, combined with solid phase extraction method was developed for the separation and determination of sugars in tobacco leaf. Separation was performed with a Hypersil NH2 analytical column and the mobile phase 75%:25% acetonitrile-water. In the range of 0.1-4 mg/mL, the method showed good linearity, with the correlation coefficients exceeding 0.99. The limits of detection of xylose, fructose, glucose, sucrose, maltose, and raffinose were 2, 2, 3, 2, 4, and 4 mu g/mL, respectively. The relative standard deviations of peak areas for these sugars were in the range of 2-3%. Recoveries of fructose, glucose, and sucrose were between 96 and 117%. Finally, determination of sugars in tobacco leaf, before and after bio-catalysis, was performed with this method.
Language英语
URL查看原文
WOS IDWOS:000237497300006
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/97621
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺); Xu GW(许国旺)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Biochem & Cell Biol Inst, Shanghai Inst Biol Sci, Shanghai, Peoples R China
3.Chuxiong Cigarette Co, Hongta Grp, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Pang, T,Bai, CM,Xu, YJ,et al. Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2006,29(9):1281-1289.
APA Pang, T.,Bai, CM.,Xu, YJ.,Xu, GW.,Yuan, ZY.,...&许国旺.(2006).Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,29(9),1281-1289.
MLA Pang, T,et al."Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 29.9(2006):1281-1289.
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