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Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection
Weng, QF; Xu, GW; Yuan, KL; Tang, P; Xu GW(许国旺); Xu GW(许国旺)
KeywordCapillary Electrophoresis Field-amplified Sample Injection Amperometric Detection Monoamines Urine
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2006-05-01
DOI10.1016/j.jchromb.2006.03.005
Volume835Issue:1-2Pages:55-61
Indexed BySCI
SubtypeArticle
Department18
Funding Project1808
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; DYNAMIC PH JUNCTION ; ELECTROKINETIC CHROMATOGRAPHY ; CATECHOLAMINES ; COLUMN ; FLUORESCENCE ; METABOLITES ; INJECTION ; SEROTONIN ; DOPAMINE
AbstractA simple, rapid and low-cost method using capillary electrophoresis coupled with field-amplified sample stacking and electrochemical detection was developed for the separation and determination of monoamines. In this present work, a systematic study of the parameters (pH value and concentration of electrophoretic buffer, composition of sample solvent, injection voltage and time) affecting separation and on-line concentration of monoamines has been performed enabling the detection sensitivity of these monoamines to be improved by 5000 times compared with the conventional electrokinetic injection. This developed method was applied to the direct analysis of these monoamines in human urine without off-line sample preconcentration. Due to the requirement for urine dilution to minimize the detrimental effects of high salt on analyte stacking, the real sensitivity improvement is about 50-fold when applying the optimized method to urine samples. In order to quantitate these monoamines accurately, internal standard calibration curves were constructed with standard monoamines in presence of salt with similar concentration as in human urine. In the method validation, the calibration curves were linear over a range of 1.0 x 10(-9) to 2.5 x 10(-8) mol/L for each monoamine and the limits of detection (signal to noise ratio of 3) for these monoamines were in the sub-nmol/L concentration range (6.0 x 10(-10) mol/L). (c) 2006 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000237369900008
Citation statistics
Cited Times:41[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/97623
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺); Xu GW(许国旺)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Weng, QF,Xu, GW,Yuan, KL,et al. Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,835(1-2):55-61.
APA Weng, QF,Xu, GW,Yuan, KL,Tang, P,许国旺,&许国旺.(2006).Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,835(1-2),55-61.
MLA Weng, QF,et al."Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 835.1-2(2006):55-61.
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