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Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection
Weng, QF; Xu, GW; Yuan, KL; Tang, P; Xu GW(许国旺); Xu GW(许国旺)
关键词Capillary Electrophoresis Field-amplified Sample Injection Amperometric Detection Monoamines Urine
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2006-05-01
DOI10.1016/j.jchromb.2006.03.005
835期:1-2页:55-61
收录类别SCI
文章类型Article
部门归属18
项目归属1808
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; DYNAMIC PH JUNCTION ; ELECTROKINETIC CHROMATOGRAPHY ; CATECHOLAMINES ; COLUMN ; FLUORESCENCE ; METABOLITES ; INJECTION ; SEROTONIN ; DOPAMINE
英文摘要A simple, rapid and low-cost method using capillary electrophoresis coupled with field-amplified sample stacking and electrochemical detection was developed for the separation and determination of monoamines. In this present work, a systematic study of the parameters (pH value and concentration of electrophoretic buffer, composition of sample solvent, injection voltage and time) affecting separation and on-line concentration of monoamines has been performed enabling the detection sensitivity of these monoamines to be improved by 5000 times compared with the conventional electrokinetic injection. This developed method was applied to the direct analysis of these monoamines in human urine without off-line sample preconcentration. Due to the requirement for urine dilution to minimize the detrimental effects of high salt on analyte stacking, the real sensitivity improvement is about 50-fold when applying the optimized method to urine samples. In order to quantitate these monoamines accurately, internal standard calibration curves were constructed with standard monoamines in presence of salt with similar concentration as in human urine. In the method validation, the calibration curves were linear over a range of 1.0 x 10(-9) to 2.5 x 10(-8) mol/L for each monoamine and the limits of detection (signal to noise ratio of 3) for these monoamines were in the sub-nmol/L concentration range (6.0 x 10(-10) mol/L). (c) 2006 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000237369900008
引用统计
被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/97623
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺); Xu GW(许国旺)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Weng, QF,Xu, GW,Yuan, KL,et al. Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,835(1-2):55-61.
APA Weng, QF,Xu, GW,Yuan, KL,Tang, P,许国旺,&许国旺.(2006).Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,835(1-2),55-61.
MLA Weng, QF,et al."Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 835.1-2(2006):55-61.
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