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On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane
Yang, BC; Zhang, FF; Tian, HZ; Guan, YF; Guan YF(关亚风); Guan YF(关亚风)
关键词Protein Preconcentration Capillary Electrophoresis Porous Membrane
刊名JOURNAL OF CHROMATOGRAPHY A
2006-06-09
DOI10.1016/j.chroma.2006.03.105
1117期:2页:214-218
收录类别SCI
文章类型Article
部门归属1
项目归属105
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]DYNAMIC PH JUNCTION ; ZONE-ELECTROPHORESIS ; ELECTROKINETIC CHROMATOGRAPHY ; MASS-SPECTROMETRY ; JOINT ; INJECTION ; PEPTIDES ; ANALYTES
英文摘要A simple on-line preconcentration method of protein for capillary electrophoresis (CE) using a cellulose acetate (CA)-coated porous membrane was proposed. CA membrane is fabricated at one of the ends of the column that allows the passage of buffer ions but excludes larger protein molecules. Protein sample is continuously electrokinetically loaded and trapped by the membrane. When injection is completed, the direction of the electric field is switched and the trapped proteins are then separated by conventional CE procedure. The results achieved showed that the preconcentration mechanism of this method was based on size-exclusion effect. Bovine serum albumin (BSA) was used for model protein sample, and signal enhancement of 550-fold with 15 min injection time was achieved. (c) 2006 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000238005800013
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被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/97631
专题中国科学院大连化学物理研究所
通讯作者Guan YF(关亚风); Guan YF(关亚风)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Yang, BC,Zhang, FF,Tian, HZ,et al. On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1117(2):214-218.
APA Yang, BC,Zhang, FF,Tian, HZ,Guan, YF,关亚风,&关亚风.(2006).On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane.JOURNAL OF CHROMATOGRAPHY A,1117(2),214-218.
MLA Yang, BC,et al."On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane".JOURNAL OF CHROMATOGRAPHY A 1117.2(2006):214-218.
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