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题名: On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane
作者: Yang, BC;  Zhang, FF;  Tian, HZ;  Guan, YF
通讯作者: 关亚风
关键词: protein preconcentration ;  capillary electrophoresis ;  porous membrane
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2006-06-09
DOI: 10.1016/j.chroma.2006.03.105
卷: 1117, 期:2, 页:214-218
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 105
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A simple on-line preconcentration method of protein for capillary electrophoresis (CE) using a cellulose acetate (CA)-coated porous membrane was proposed. CA membrane is fabricated at one of the ends of the column that allows the passage of buffer ions but excludes larger protein molecules. Protein sample is continuously electrokinetically loaded and trapped by the membrane. When injection is completed, the direction of the electric field is switched and the trapped proteins are then separated by conventional CE procedure. The results achieved showed that the preconcentration mechanism of this method was based on size-exclusion effect. Bovine serum albumin (BSA) was used for model protein sample, and signal enhancement of 550-fold with 15 min injection time was achieved. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: DYNAMIC PH JUNCTION ;  ZONE-ELECTROPHORESIS ;  ELECTROKINETIC CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  JOINT ;  INJECTION ;  PEPTIDES ;  ANALYTES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000238005800013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97631
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Yang, BC,Zhang, FF,Tian, HZ,et al. On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1117(2):214-218.
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