DICP OpenIR
A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems
Xu, J; Yang, BC; Tian, HZ; Guan, YF; Guan YF(关亚风); Guan YF(关亚风)
KeywordFluorescence Detector Windowless Flow Cell Light-emitting Diode Capillary Hplc Flow Injection
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2006-04-01
DOI10.1007/s00216-006-0351-3
Volume384Issue:7-8Pages:1590-1593
Indexed BySCI
SubtypeArticle
Department1
Funding Project105
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordLIGHT-EMITTING-DIODE ; WAVE-GUIDE ; FLUOROMETRIC DETECTION ; OPTICAL-FIBER ; ELECTROPHORESIS ; INJECTION ; SEPARATION ; SAMPLE
AbstractA miniaturized fluorescence detector utilizing a three-dimensional windowless flow cell has been constructed and evaluated. The inlet and outlet liquid channels are collinear and are located in the same plane as the excitation paths, while the optical fiber used to collect the emission light is perpendicular to this plane. The straightforward arrangement of the flow path minimizes band dispersion and eliminates bubble formation or accumulation inside the cell. The use of high-brightness light-emitting diodes (LEDs) as the excitation source and a miniaturized metal package photomultiplier tube (PMT) results in a compact and sensitive fluorescence detector. The detection limit obtained from the system for fluorescein isothiocyanate (FITC) in flow injection mode is 2.6 nmol/L. The analysis of riboflavin and FITC by packed capillary liquid chromatography is demonstrated.
Language英语
URL查看原文
WOS IDWOS:000236502600019
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/97643
Collection中国科学院大连化学物理研究所
Corresponding AuthorGuan YF(关亚风); Guan YF(关亚风)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Xu, J,Yang, BC,Tian, HZ,et al. A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,384(7-8):1590-1593.
APA Xu, J,Yang, BC,Tian, HZ,Guan, YF,关亚风,&关亚风.(2006).A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,384(7-8),1590-1593.
MLA Xu, J,et al."A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 384.7-8(2006):1590-1593.
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