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题名: A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems
作者: Xu, J;  Yang, BC;  Tian, HZ;  Guan, YF
通讯作者: 关亚风
关键词: fluorescence detector ;  windowless flow cell ;  light-emitting diode ;  capillary HPLC ;  flow injection
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2006-04-01
DOI: 10.1007/s00216-006-0351-3
卷: 384, 期:7-8, 页:1590-1593
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 105
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A miniaturized fluorescence detector utilizing a three-dimensional windowless flow cell has been constructed and evaluated. The inlet and outlet liquid channels are collinear and are located in the same plane as the excitation paths, while the optical fiber used to collect the emission light is perpendicular to this plane. The straightforward arrangement of the flow path minimizes band dispersion and eliminates bubble formation or accumulation inside the cell. The use of high-brightness light-emitting diodes (LEDs) as the excitation source and a miniaturized metal package photomultiplier tube (PMT) results in a compact and sensitive fluorescence detector. The detection limit obtained from the system for fluorescein isothiocyanate (FITC) in flow injection mode is 2.6 nmol/L. The analysis of riboflavin and FITC by packed capillary liquid chromatography is demonstrated.
关键词[WOS]: LIGHT-EMITTING-DIODE ;  WAVE-GUIDE ;  FLUOROMETRIC DETECTION ;  OPTICAL-FIBER ;  ELECTROPHORESIS ;  INJECTION ;  SEPARATION ;  SAMPLE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000236502600019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97643
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Xu, J,Yang, BC,Tian, HZ,et al. A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,384(7-8):1590-1593.
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