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A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems
Xu, J; Yang, BC; Tian, HZ; Guan, YF; Guan YF(关亚风); Guan YF(关亚风)
关键词Fluorescence Detector Windowless Flow Cell Light-emitting Diode Capillary Hplc Flow Injection
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2006-04-01
DOI10.1007/s00216-006-0351-3
384期:7-8页:1590-1593
收录类别SCI
文章类型Article
部门归属1
项目归属105
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]LIGHT-EMITTING-DIODE ; WAVE-GUIDE ; FLUOROMETRIC DETECTION ; OPTICAL-FIBER ; ELECTROPHORESIS ; INJECTION ; SEPARATION ; SAMPLE
英文摘要A miniaturized fluorescence detector utilizing a three-dimensional windowless flow cell has been constructed and evaluated. The inlet and outlet liquid channels are collinear and are located in the same plane as the excitation paths, while the optical fiber used to collect the emission light is perpendicular to this plane. The straightforward arrangement of the flow path minimizes band dispersion and eliminates bubble formation or accumulation inside the cell. The use of high-brightness light-emitting diodes (LEDs) as the excitation source and a miniaturized metal package photomultiplier tube (PMT) results in a compact and sensitive fluorescence detector. The detection limit obtained from the system for fluorescein isothiocyanate (FITC) in flow injection mode is 2.6 nmol/L. The analysis of riboflavin and FITC by packed capillary liquid chromatography is demonstrated.
语种英语
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WOS记录号WOS:000236502600019
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/97643
专题中国科学院大连化学物理研究所
通讯作者Guan YF(关亚风); Guan YF(关亚风)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Xu, J,Yang, BC,Tian, HZ,et al. A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,384(7-8):1590-1593.
APA Xu, J,Yang, BC,Tian, HZ,Guan, YF,关亚风,&关亚风.(2006).A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,384(7-8),1590-1593.
MLA Xu, J,et al."A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 384.7-8(2006):1590-1593.
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