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题名: Preparation and application of monolithic columns with narrow immobilized pH gradients
作者: Zhu, Guijie;  Yang, Chun;  Zhang, Lihua;  Liang, Zhen;  Zhang, Weibing;  Zhang, Yukui
通讯作者: 张丽华
关键词: monolithic immobilized pH gradient ;  column preparation ;  narrow pH gradient
刊名: TALANTA
发表日期: 2006-08-15
DOI: 10.1016/j.talanta.2005.12.037
卷: 70, 期:1, 页:2-6
收录类别: ISTP ;  SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Monolith with immobilized pH gradient (M-IPG) is a novel separation matrix for amphoteric substances, such as peptides and proteins. To improve the properties of the newly designed column, efforts were made to optimize the preparation procedure, including the concentrations of the monomers, glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EDMA), as well as the kinds of porogens, which led to a monolith with improved permeability, uniformity and continuity. In addition, different diamines, including ethylenediamine, 1,3-diaminopropane, 1,4-diaminobutane and 1,5-diaminopentane, were subjected to aminate the polymer, and the last two exhibited excellent reactivity with epoxy on the polymer surface, which could obviously make the immobilization of pH gradient facilitated. Under the optimal conditions, a simple method to prepare M-IPG column with narrow pH gradient was developed with commercial carrier ampholytes (CAs) solution. Such columns were applied into the analysis of proteins and peptides, and showed the improved resolution compared to the traditional one with a wide pH distribution. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: CAPILLARY ELECTROCHROMATOGRAPHY ;  CARRIER AMPHOLYTES ;  STATIONARY PHASES ;  ELECTROPHORESIS ;  STATE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000239709400002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97681
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhu, Guijie,Yang, Chun,Zhang, Lihua,et al. Preparation and application of monolithic columns with narrow immobilized pH gradients[J]. TALANTA,2006,70(1):2-6.
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