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题名: New luminescent europium(III) chelates for DNA labeling
作者: Nishioka, T;  Yuan, JL;  Yamamoto, Y;  Sumitomo, K;  Wang, Z;  Hashino, K;  Hosoya, C;  Ikawa, K;  Wang, GL;  Matsumoto, K
通讯作者: Kazuko Matsumoto
刊名: INORGANIC CHEMISTRY
发表日期: 2006-05-15
DOI: 10.1021/ic051276g
卷: 45, 期:10, 页:4088-4096
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 101
产权排名: 3;2
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Inorganic & Nuclear
研究领域[WOS]: Chemistry
英文摘要: The new europium(III) chelate {2,2', 2", 2"'-{[4'-(aminobiphenyl-4-yl)-2,2': 6',2"-terpyridine-6,6"-diyl]bis(methylenenitrilo)}-tetrakis( acetato)} europium(III) (ATBTA-Eu3+) and its 4,6-dichloro-1,3,5-triazinyl and succinimidyl derivatives ( DTBTA and NHS-ATBTA, respectively) were synthesized and characterized. Both labeling complexes DTBTA-Eu3+ and NHS-ATBTA-Eu3+ are luminescent. Especially DTBTA-Eu3+ is strongly luminescent, with a luminescence quantum yield of 9.1%, molar extinction coefficient of 3.1 x 104 cm(-1) M-1 ( 335 nm), and luminescence lifetime of 1.02 ms. The excitation and emission maximum wavelengths of DTBTA-Eu3+ are 335 and 616 nm, respectively. The complex is very stable in aqueous buffers, with a conditional formation constant log KDTBTA-Eu of 25.0 at pH 8, and can be conjugated to DNA and proteins. The chelates are also highly resistant to thermal decomposition, photodegradation, and ozone oxidation. These properties prove that DTBTA-Eu3+ is suitable as a luminescence label in DNA assays.
关键词[WOS]: TIME-RESOLVED FLUOROIMMUNOASSAY ;  STATE ENERGY-LEVEL ;  LANTHANIDE COMPLEXES ;  FLUORESCENT CHELATE ;  BIOANALYTICAL ASSAYS ;  AQUEOUS-SOLUTION ;  DERIVATIVES ;  BINDING ;  AGENTS ;  2,2'/6',2''-TERPYRIDINE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000237593100038
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97919
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan
2.Waseda Univ, Adv Res Inst Sci & Engn, Shinjuku Ku, Tokyo 1698555, Japan
3.Chinese Acad Sci, Dept Analyt Chem, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
4.Japan Sci & Technol Agcy, Kawaguchi, Japan

Recommended Citation:
Nishioka, T,Yuan, JL,Yamamoto, Y,et al. New luminescent europium(III) chelates for DNA labeling[J]. INORGANIC CHEMISTRY,2006,45(10):4088-4096.
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