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题名: A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe
作者: Tan, MQ;  Song, B;  Wang, GL;  Yuan, JL
通讯作者: 袁景利
关键词: terbium ;  fluorescence probe ;  singlet oxygen ;  time-resolved fluorescence detection ;  free radicals
刊名: FREE RADICAL BIOLOGY AND MEDICINE
发表日期: 2006-05-01
DOI: 10.1016/j.freeradbiomed.2005.12.030
卷: 40, 期:9, 页:1644-1653
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 101
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Endocrinology & Metabolism
研究领域[WOS]: Biochemistry & Molecular Biology ;  Endocrinology & Metabolism
英文摘要: A terbium(III) chelate fluorescence probe for detection of singlet oxygen (102) in aqueous media, NNN',N-[2,6-bis(3'-aminomethyl-l'pyrazolyi)-4-(9"-anthryl)pyridine] tetrakis (acetate)-Tb3+ (PATA-Tb3+), was designed and synthesized. The new chelate is highly water soluble, is almost nonfluorescent, and can specifically react with O-1(2) to yield a strongly fluorescent chelate, the endoperoxide of PATA-Tb3+, accompanied by a remarkable increase in the fluorescence quantum yield from 0.46 to 10.5%. The long fluorescence lifetime of the endoperoxide (2.76 ms) allows the probe to be used favorably for time-resolved fluorescence detection Of O-1(2). The studies of fluorescence property and reaction specificity indicate that the new probe is highly sensitive and selective for O-1(2). The probe was used for quantitative detection Of O-1(2) generated from a MoO42--H2O2 system to give a detection limit of 10.8 nM. In addition, the good applicability of the probe was demonstrated by the real-time monitoring of the kinetic process of O-1(2) generation in a horseradish peroxidase-catalyzed oxidation system of indole-3-acetic acid in a weakly acidic buffer. (c) 2006 Elsevier Inc. All rights reserved.
关键词[WOS]: PEROXIDASE-CATALYZED OXIDATION ;  HYDROGEN-PEROXIDE ;  MOLECULAR-OXYGEN ;  BIOANALYTICAL ASSAYS ;  PHOTODYNAMIC THERAPY ;  RATIONAL DESIGN ;  MURINE TUMOR ;  GENERATION ;  SENSITIZER ;  IDENTIFICATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000237433900016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97921
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dept Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Tan, MQ,Song, B,Wang, GL,et al. A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe[J]. FREE RADICAL BIOLOGY AND MEDICINE,2006,40(9):1644-1653.
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