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Synthesis and characterization of titania-based monodisperse fluorescent europium nanoparticles for biolabeling
Tan, MQ; Wang, GL; Ye, ZQ; Yuan, JL; Yuan JL(袁景利); Yuan JL(袁景利)
关键词Europium Fluorescence Nanoparticle Titanium Oxide Biolabeling Material
刊名JOURNAL OF LUMINESCENCE
2006-03-01
ISSN0022-2313
DOI10.1016/j.jlumin.2005.04.004
117期:1页:20-28
收录类别SCI
文章类型Article
部门归属1
项目归属101
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Optics
研究领域[WOS]Optics
关键词[WOS]RESOLVED FLUOROMETRIC APPLICATION ; PROSTATE-SPECIFIC ANTIGEN ; FUNCTIONALIZED SILICA-GEL ; LUMINESCENCE PROPERTIES ; TIO2 NANOPARTICLES ; HYBRID MATERIALS ; THIN-FILMS ; COMPLEXES ; PARTICLES ; COMPOSITE
英文摘要Inorganic-organic hybrid titania-based nanoparticles covalently bound to a fluorescent Eu3+ chelate of 4,4'-bis(1 '',1 '',1 '', 2 '',2 ',3 '',3 ''-heptafluoro-4 '',6 ''-hexanedion-6 ''-yl)chlorosulfo-o-terphenyl (BHHCT-Eu3+) were synthesized by a sol-gel technique. A conjugate of BHHCT with 3-[2-(2-aminoethylamino) ethylamino]propyl-trimethoxysilane (APTS) was used as a precursor for the nanoparticle preparation and monodisperse nanoparticles consisting of titania network and silica sub-network covalently bound to the Eu3+ chelate were prepared by the copolymerization of APTS-BHHCT conjugate, titanium tetraisopropoxide (TTIP) and free APTS in EuCl3 water-alcohol solution. The effects of reaction conditions on size and fluorescence lifetime of the nanoparticles were investigated. The characterizations by transmission electron microscopy and fluorometric methods indicate that the nanoparticles are near spherical and strongly fluorescent having a fluorescence quantum yield of 11.6% and a long fluorescence lifetime of similar to 0.4ms. The direct-introduced amino groups on the nanoparticle's surface by using free APTS in nanoparticle preparation facilitated the biolabeling process of the nanoparticles. The nanoparticle-labeled streptavidin (SA) was prepared and used in a sandwich-type time-resolved fluoroimmunoassay (TR-FIA) of human prostate-specific antigen (PSA) by using a 96-well microtiter plate as the solid phase carrier. The method gives a detection limit of 66 pg/ml for the PSA assay. (c) 2005 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000234357700003
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被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/97927
专题中国科学院大连化学物理研究所
通讯作者Yuan JL(袁景利); Yuan JL(袁景利)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Grad Sch, Dalian 116023, Peoples R China
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Tan, MQ,Wang, GL,Ye, ZQ,et al. Synthesis and characterization of titania-based monodisperse fluorescent europium nanoparticles for biolabeling[J]. JOURNAL OF LUMINESCENCE,2006,117(1):20-28.
APA Tan, MQ,Wang, GL,Ye, ZQ,Yuan, JL,袁景利,&袁景利.(2006).Synthesis and characterization of titania-based monodisperse fluorescent europium nanoparticles for biolabeling.JOURNAL OF LUMINESCENCE,117(1),20-28.
MLA Tan, MQ,et al."Synthesis and characterization of titania-based monodisperse fluorescent europium nanoparticles for biolabeling".JOURNAL OF LUMINESCENCE 117.1(2006):20-28.
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