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题名: Synthesis and characterization of titania-based monodisperse fluorescent europium nanoparticles for biolabeling
作者: Tan, MQ;  Wang, GL;  Ye, ZQ;  Yuan, JL
通讯作者: 袁景利
关键词: europium ;  fluorescence ;  nanoparticle ;  titanium oxide ;  biolabeling material
刊名: JOURNAL OF LUMINESCENCE
发表日期: 2006-03-01
DOI: 10.1016/j.jlumin.2005.04.004
卷: 117, 期:1, 页:20-28
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 101
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Optics
研究领域[WOS]: Optics
英文摘要: Inorganic-organic hybrid titania-based nanoparticles covalently bound to a fluorescent Eu3+ chelate of 4,4'-bis(1 '',1 '',1 '', 2 '',2 ',3 '',3 ''-heptafluoro-4 '',6 ''-hexanedion-6 ''-yl)chlorosulfo-o-terphenyl (BHHCT-Eu3+) were synthesized by a sol-gel technique. A conjugate of BHHCT with 3-[2-(2-aminoethylamino) ethylamino]propyl-trimethoxysilane (APTS) was used as a precursor for the nanoparticle preparation and monodisperse nanoparticles consisting of titania network and silica sub-network covalently bound to the Eu3+ chelate were prepared by the copolymerization of APTS-BHHCT conjugate, titanium tetraisopropoxide (TTIP) and free APTS in EuCl3 water-alcohol solution. The effects of reaction conditions on size and fluorescence lifetime of the nanoparticles were investigated. The characterizations by transmission electron microscopy and fluorometric methods indicate that the nanoparticles are near spherical and strongly fluorescent having a fluorescence quantum yield of 11.6% and a long fluorescence lifetime of similar to 0.4ms. The direct-introduced amino groups on the nanoparticle's surface by using free APTS in nanoparticle preparation facilitated the biolabeling process of the nanoparticles. The nanoparticle-labeled streptavidin (SA) was prepared and used in a sandwich-type time-resolved fluoroimmunoassay (TR-FIA) of human prostate-specific antigen (PSA) by using a 96-well microtiter plate as the solid phase carrier. The method gives a detection limit of 66 pg/ml for the PSA assay. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: RESOLVED FLUOROMETRIC APPLICATION ;  PROSTATE-SPECIFIC ANTIGEN ;  FUNCTIONALIZED SILICA-GEL ;  LUMINESCENCE PROPERTIES ;  TIO2 NANOPARTICLES ;  HYBRID MATERIALS ;  THIN-FILMS ;  COMPLEXES ;  PARTICLES ;  COMPOSITE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000234357700003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/97927
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Grad Sch, Dalian 116023, Peoples R China

Recommended Citation:
Tan, MQ,Wang, GL,Ye, ZQ,et al. Synthesis and characterization of titania-based monodisperse fluorescent europium nanoparticles for biolabeling[J]. JOURNAL OF LUMINESCENCE,2006,117(1):20-28.
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