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Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells
Liang, Aiye; Zhou, Xiaomian; Wang, Qiuyan; Liu, Xiu; Qin, Jianhua; Du, Yuguang; Wang, Keyi; Lin, Bingcheng; Lin BC(林炳承); Lin BC(林炳承)
关键词Capillary Zone Electrophoresis Dextran Sulfate Granulocyte Colony-stimulating Factor Interaction Leukemia Cells
刊名ELECTROPHORESIS
2006-08-01
DOI10.1002/elps.200500758
27期:16页:3195-3201
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]FIBROBLAST GROWTH-FACTOR ; HEPARAN-SULFATE ; CAPILLARY-ELECTROPHORESIS ; BINDING ; PROTEOGLYCANS ; DIFFERENTIATION ; RECEPTOR ; COMPLEX ; PROTEIN ; NFS-60
英文摘要The interactions between granulocyte colony-stimulating factor (G-CSF) and dextran sulfate (DS) with different chain lengths and sulfate contents were studied by capillary zone electrophoresis. It was found that DS with a molecular mass of 500 kDa (DS500) could bind to G-CSF and the binding constant and binding sites were determined using Scatchard plot to be 1.17 x 10(6) M(-1) and 8, respectively. DS with a molecular mass of 40 kDa also had the affinity to G-CSF and the binding constant and binding sties were 1.01 x 10(6) M-1 and 8, respectively. However, DS with a molecular mass of 8 kDa and the non-sulfated saccharide, dextran, had no affinity to G-CSF The results indicate that the interactions between G-CSIF and DS are dependent on the chain lengths and sulfate contents of the saccharides. In addition, the effects of G-CSF-binding DS on a G-CSF-dependent leukemia cell line were investigated using biological methods. Results show that DS500 plus G-CSF has potential therapeutic effect on cancers because their combination could inhibit the growth and induce the differentiation of the leukemia cells.
语种英语
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WOS记录号WOS:000240333600005
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98071
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承); Lin BC(林炳承)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.First Municipal Peoples Hosp, Guangzhou, Peoples R China
4.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China
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GB/T 7714
Liang, Aiye,Zhou, Xiaomian,Wang, Qiuyan,et al. Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells[J]. ELECTROPHORESIS,2006,27(16):3195-3201.
APA Liang, Aiye.,Zhou, Xiaomian.,Wang, Qiuyan.,Liu, Xiu.,Qin, Jianhua.,...&林炳承.(2006).Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells.ELECTROPHORESIS,27(16),3195-3201.
MLA Liang, Aiye,et al."Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells".ELECTROPHORESIS 27.16(2006):3195-3201.
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