中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells
作者: Liang, Aiye;  Zhou, Xiaomian;  Wang, Qiuyan;  Liu, Xiu;  Qin, Jianhua;  Du, Yuguang;  Wang, Keyi;  Lin, Bingcheng
通讯作者: 林炳承
关键词: capillary zone electrophoresis ;  dextran sulfate ;  granulocyte colony-stimulating factor ;  interaction ;  leukemia cells
刊名: ELECTROPHORESIS
发表日期: 2006-08-01
DOI: 10.1002/elps.200500758
卷: 27, 期:16, 页:3195-3201
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The interactions between granulocyte colony-stimulating factor (G-CSF) and dextran sulfate (DS) with different chain lengths and sulfate contents were studied by capillary zone electrophoresis. It was found that DS with a molecular mass of 500 kDa (DS500) could bind to G-CSF and the binding constant and binding sites were determined using Scatchard plot to be 1.17 x 10(6) M(-1) and 8, respectively. DS with a molecular mass of 40 kDa also had the affinity to G-CSF and the binding constant and binding sties were 1.01 x 10(6) M-1 and 8, respectively. However, DS with a molecular mass of 8 kDa and the non-sulfated saccharide, dextran, had no affinity to G-CSF The results indicate that the interactions between G-CSIF and DS are dependent on the chain lengths and sulfate contents of the saccharides. In addition, the effects of G-CSF-binding DS on a G-CSF-dependent leukemia cell line were investigated using biological methods. Results show that DS500 plus G-CSF has potential therapeutic effect on cancers because their combination could inhibit the growth and induce the differentiation of the leukemia cells.
关键词[WOS]: FIBROBLAST GROWTH-FACTOR ;  HEPARAN-SULFATE ;  CAPILLARY-ELECTROPHORESIS ;  BINDING ;  PROTEOGLYCANS ;  DIFFERENTIATION ;  RECEPTOR ;  COMPLEX ;  PROTEIN ;  NFS-60
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000240333600005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98071
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.First Municipal Peoples Hosp, Guangzhou, Peoples R China
4.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China

Recommended Citation:
Liang, Aiye,Zhou, Xiaomian,Wang, Qiuyan,et al. Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells[J]. ELECTROPHORESIS,2006,27(16):3195-3201.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Liang, Aiye]'s Articles
 [Zhou, Xiaomian]'s Articles
 [Wang, Qiuyan]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Liang, Aiye]‘s Articles
 [Zhou, Xiaomian]‘s Articles
 [Wang, Qiuyan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace