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题名: Studying drug-plasma protein interactions by two-injector microchip electrophoresis frontal analysis
作者: Liu, Xiaojun;  Liang, Aiye;  Shen, Zheng;  Liu, Xin;  Zhang, Yu;  Dai, Zhongpeng;  Xiong, Bohui;  Lin, Bingcheng
通讯作者: 林炳承
关键词: drug-plasma protein interactions ;  frontal analysis ;  microchip electrophoresis
刊名: ELECTROPHORESIS
发表日期: 2006-12-01
DOI: 10.1002/elps.200600294
卷: 27, 期:24, 页:5128-5131
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: We developed a simple, rapid, and sensitive two-injector microchip electrophoresis frontal analysis (MCE-FA) method for studying drug-plasma protein interactions. In this method, large volumes of a reference sample and drug-plasma protein mixture were simultaneously introduced into the respective sections of the microchannel through the separated injectors and then electrophoresed. Since the reference sample did not meet with the interacting species during migration, it could be used as an external standard. The interaction between heparin and HSA was quantitatively characterized as a model system. The binding constant was found to be (1.53 +/- 0.01) X 10(4) M-1.
关键词[WOS]: AFFINITY CAPILLARY-ELECTROPHORESIS ;  INDUCED FLUORESCENCE DETECTION ;  AMYLOID P COMPONENT ;  ZONE-ELECTROPHORESIS ;  BINDING ;  HEPARIN ;  DNA ;  METHODOLOGY ;  PEPTIDE ;  ASSAY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000243304100030
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98081
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 116023, Peoples R China

Recommended Citation:
Liu, Xiaojun,Liang, Aiye,Shen, Zheng,et al. Studying drug-plasma protein interactions by two-injector microchip electrophoresis frontal analysis[J]. ELECTROPHORESIS,2006,27(24):5128-5131.
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