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题名: Isolation and characterization of angiotensin I-converting enzyme inhibitory peptides derived from porcine hemoglobin
作者: Yu, Yike;  Hu, Jianen;  Miyaguchi, Yuji;  Bai, Xuefang;  Du, Yuguang;  Lin, Bingcheng
通讯作者: 杜昱光
关键词: angiotensin I-converting enzyme ;  inhibitory activity ;  peptide ;  porcine hemoglobin
刊名: PEPTIDES
发表日期: 2006-11-01
DOI: 10.1016/j.peptides.2006.05.025
卷: 27, 期:11, 页:2950-2956
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1805,1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ;  Pharmacology & Pharmacy
英文摘要: Animal blood is potentially an untapped source of drugs and value-added food production. More than 400 million pigs are slaughtered each year but porcine blood is usually discarded in China. This study describes the isolation and characterization of angiotensin I-converting enzyme (ACE) inhibitory peptides derived from porcine hemoglobin. The most active hydrolysate was obtained from the peptic digestion of porcine hemoglobin. After the purification of ACE-inhibitory peptides with Sephadex LH-20 gel chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC) on C-18 column, two active fractions were obtained. They were analyzed by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS). They were LGFPTTKTYFPHF and VVYPWT, corresponding to the 34-46 fragment of the a chain and the 34-39 fragment of the a chain of porcine hemoglobin, with IC50 values of 4.92 and 6.02 wM, respectively. They were the first found from porcine hemoglobin; in particular, LGFPTTKTYFPHF was a novel ACE-inhibitory peptide. In addition, the purified ACE inhibitors both competitively inhibited ACE, and maintained inhibitory activity even after incubation with gastrointestinal proteases. This suggests that these peptides might have a potential antihypertensive effect. (c) 2006 Elsevier Inc. All rights reserved.
关键词[WOS]: OLIGOPEPTIDE-ENRICHED HYDROLYSATE ;  WHEY-PROTEIN DIGESTS ;  GLOBIN ;  FOOD
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000242318900051
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98153
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Ibaraki Univ, Coll Agr, Ibaraki 3000393, Japan

Recommended Citation:
Yu, Yike,Hu, Jianen,Miyaguchi, Yuji,et al. Isolation and characterization of angiotensin I-converting enzyme inhibitory peptides derived from porcine hemoglobin[J]. PEPTIDES,2006,27(11):2950-2956.
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