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Isolation and characterization of angiotensin I-converting enzyme inhibitory peptides derived from porcine hemoglobin
Yu, Yike; Hu, Jianen; Miyaguchi, Yuji; Bai, Xuefang; Du, Yuguang; Lin, Bingcheng; Du YG(杜昱光); Du YG(杜昱光)
关键词Angiotensin I-converting Enzyme Inhibitory Activity Peptide Porcine Hemoglobin
刊名PEPTIDES
2006-11-01
DOI10.1016/j.peptides.2006.05.025
27期:11页:2950-2956
收录类别SCI
文章类型Article
部门归属18
项目归属1805,1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]OLIGOPEPTIDE-ENRICHED HYDROLYSATE ; WHEY-PROTEIN DIGESTS ; GLOBIN ; FOOD
英文摘要Animal blood is potentially an untapped source of drugs and value-added food production. More than 400 million pigs are slaughtered each year but porcine blood is usually discarded in China. This study describes the isolation and characterization of angiotensin I-converting enzyme (ACE) inhibitory peptides derived from porcine hemoglobin. The most active hydrolysate was obtained from the peptic digestion of porcine hemoglobin. After the purification of ACE-inhibitory peptides with Sephadex LH-20 gel chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC) on C-18 column, two active fractions were obtained. They were analyzed by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS). They were LGFPTTKTYFPHF and VVYPWT, corresponding to the 34-46 fragment of the a chain and the 34-39 fragment of the a chain of porcine hemoglobin, with IC50 values of 4.92 and 6.02 wM, respectively. They were the first found from porcine hemoglobin; in particular, LGFPTTKTYFPHF was a novel ACE-inhibitory peptide. In addition, the purified ACE inhibitors both competitively inhibited ACE, and maintained inhibitory activity even after incubation with gastrointestinal proteases. This suggests that these peptides might have a potential antihypertensive effect. (c) 2006 Elsevier Inc. All rights reserved.
语种英语
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WOS记录号WOS:000242318900051
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被引频次:53[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98153
专题中国科学院大连化学物理研究所
通讯作者Du YG(杜昱光); Du YG(杜昱光)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Ibaraki Univ, Coll Agr, Ibaraki 3000393, Japan
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Yu, Yike,Hu, Jianen,Miyaguchi, Yuji,et al. Isolation and characterization of angiotensin I-converting enzyme inhibitory peptides derived from porcine hemoglobin[J]. PEPTIDES,2006,27(11):2950-2956.
APA Yu, Yike.,Hu, Jianen.,Miyaguchi, Yuji.,Bai, Xuefang.,Du, Yuguang.,...&杜昱光.(2006).Isolation and characterization of angiotensin I-converting enzyme inhibitory peptides derived from porcine hemoglobin.PEPTIDES,27(11),2950-2956.
MLA Yu, Yike,et al."Isolation and characterization of angiotensin I-converting enzyme inhibitory peptides derived from porcine hemoglobin".PEPTIDES 27.11(2006):2950-2956.
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