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A spectroscopic study on the interactions of porphyrin with G-quadruplex DNAs
Wei, Chunying; Jia, Guoqing; Yuan, Jingli; Feng, Zhaochi; Li, Can; Li C(李灿); Li C(李灿)
刊名BIOCHEMISTRY
2006-05-30
DOI10.1021/bi052356z
45期:21页:6681-6691
收录类别SCI
文章类型Article
部门归属5
项目归属503
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]RESONANCE RAMAN-SPECTROSCOPY ; STRANDED GUANINE TETRAPLEX ; OXYTRICHA TELOMERIC DNA ; SURFACE-ENHANCED RAMAN ; CRYSTAL-STRUCTURE ; CATIONIC PORPHYRINS ; NUCLEIC-ACIDS ; SELECTIVE INTERACTIONS ; LIGAND COMPLEX ; B-DNA
英文摘要Free-base porphyrin (5,10,15,20-tetrakis(1-methyl-4-pyridyl)-21H,23H-porphine) (H(2)TMPyP4) has been shown to be an effective telomerase inhibitor by an in vitro assay. Here, we examined the interactions of the H(2)TMPyP4 with three distinct G-quadruplex DNAs, the parallel-stranded (TG(4)T)4, dimer-hairpin-folded (G(4)T(4)G(4))2, and monomer- folded AG(3)(T(2)AG(3))(3), by ultraviolet resonance Raman spectroscopy (UVRR), UV-vis absorption spectroscopy, fluorescence spectroscopy, and surface-enhanced Raman spectroscopy (SERS). The data obtained by the continuous variation titration method show that the binding stoichiometry of H(2)TMPyP4/G-quadruplex is 2:1 for (TG(4)T)4 and 4:1 for (G(4)T(4)G(4))2 or AG(3)(T(2)AG(3))(3). The results of SERS spectra, UV-vis absorption titration, and fluorescence emission spectra together with the binding stoichiometries reveal that two H2TMPyP4 molecules are externally stacked at two ends of the parallel (TG(4)T)4 G-quadruplex, whereas H(2)TMPyP4 molecules can intercalate within their diagonal or lateral loop regions and intervals between two G-tetrads for (G(4)T(4)G(4))2 and AG(3)(T(2)AG(3))(3) G-quadruplexes. The binding of H(2)TMPyP4 to (TG(4)T)4 G-quadruplex results in the hypochromicity of the UV Raman signal of (TG(4)T)4, indicating that the stacking effects between H2TMPyP4 and DNA bases are significant. The Raman hyperchromicities and shifts are observed after the binding of H(2)TMPyP4 to both (G(4)T(4)G(4))2 and AG(3)(T(2)AG(3))(3) G-quadruplexes. This indicates that the intercalative H(2)TMPyP4 can lengthen the vertical distance between adjacent G-tetrads of (G(4)T(4)G(4))2 and AG(3)(T(2)AG(3))(3) and change their conformations. The present study provides new insights into the effect of H(2)TMPyP4 binding on the structures of G-quadruplexes and also demonstrates that Raman spectroscopy is an ideal method for examining the interaction between drugs and G-quadruplexes.
语种英语
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WOS记录号WOS:000237721700015
引用统计
被引频次:184[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98239
专题中国科学院大连化学物理研究所
通讯作者Li C(李灿); Li C(李灿)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Analyt Chem Lab, Dalian 116023, Peoples R China
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GB/T 7714
Wei, Chunying,Jia, Guoqing,Yuan, Jingli,et al. A spectroscopic study on the interactions of porphyrin with G-quadruplex DNAs[J]. BIOCHEMISTRY,2006,45(21):6681-6691.
APA Wei, Chunying.,Jia, Guoqing.,Yuan, Jingli.,Feng, Zhaochi.,Li, Can.,...&李灿.(2006).A spectroscopic study on the interactions of porphyrin with G-quadruplex DNAs.BIOCHEMISTRY,45(21),6681-6691.
MLA Wei, Chunying,et al."A spectroscopic study on the interactions of porphyrin with G-quadruplex DNAs".BIOCHEMISTRY 45.21(2006):6681-6691.
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