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题名: Isolation of a novel Ser/Thr protein kinase gene from oligochitosan-induced tobacco and its role in resistance against tobacco mosaic virus
作者: Feng, B.;  Chen, Y.;  Zhao, C.;  Zhao, X.;  Bai, X.;  Du, Y.
通讯作者: 冯斌
关键词: oligochitosan ;  Ser/Thr protein kinase ;  PAL ;  TMV resistance ;  transgenic tobacco
刊名: PLANT PHYSIOLOGY AND BIOCHEMISTRY
发表日期: 2006-10-01
DOI: 10.1016/j.plaphy.2006.10.003
卷: 44, 期:10, 页:596-603
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1805
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Plant Sciences
研究领域[WOS]: Plant Sciences
英文摘要: Oligochitosan induces defense responses to pathogenic microbes in a wide variety of plants by acting as an elicitor. In the present study, mRNA differential display was used to investigate oligochitosan-induced transcriptional activation of defense-related genes. Accordingly, a novel Ser/Thr protein kinase gene was isolated and designated as oligochitosan-induced protein kinase (oipk). Molecular cloning showed that oipk contains six introns interrupted by seven exons. The open reading frame (ORF) of the gene is 1848 bp, which encodes a putative protein of 615 amino acids with the predicted molecular mass of 70.96 kDa and a pI of 6.32. A plant oipk antisense expression vector was constructed and transformed into tobacco by Agrobacterium tumefaciens. Decreased phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activity and decreased resistance to tobacco mosaic virus (TMV) were observed in transgenic tobacco. RT-PCR analysis revealed that oipk was expressed at high levels after oligochitosan induction in wild-type tobacco, but not in transgenic tobacco. These results indicated that oipk is involved in the signal pathway of oligochitosan-induced resistance in tobacco. (c) 2006 Elsevier Masson SAS. All rights reserved.
关键词[WOS]: HIGH-AFFINITY BINDING ;  N-ACETYLCHITOOLIGOSACCHARIDE ELICITOR ;  CULTURED RICE CELLS ;  SYSTEMIC ACQUIRED-RESISTANCE ;  PHENYLALANINE AMMONIA-LYASE ;  DISEASE RESISTANCE ;  DEFENSE RESPONSES ;  PLASMA-MEMBRANE ;  SIGNAL-TRANSDUCTION ;  CHITIN FRAGMENTS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000242710500011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98331
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Liaoning Normal Univ, Coll Life Sci, Dalian 116029, Peoples R China

Recommended Citation:
Feng, B.,Chen, Y.,Zhao, C.,et al. Isolation of a novel Ser/Thr protein kinase gene from oligochitosan-induced tobacco and its role in resistance against tobacco mosaic virus[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2006,44(10):596-603.
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