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Isolation of a novel Ser/Thr protein kinase gene from oligochitosan-induced tobacco and its role in resistance against tobacco mosaic virus
Feng, B.; Chen, Y.; Zhao, C.; Zhao, X.; Bai, X.; Du, Y.; Feng B(冯斌)
关键词Oligochitosan Ser/thr Protein Kinase Pal Tmv Resistance Transgenic Tobacco
刊名PLANT PHYSIOLOGY AND BIOCHEMISTRY
2006-10-01
DOI10.1016/j.plaphy.2006.10.003
44期:10页:596-603
收录类别SCI
文章类型Article
部门归属18
项目归属1805
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Plant Sciences
研究领域[WOS]Plant Sciences
关键词[WOS]HIGH-AFFINITY BINDING ; N-ACETYLCHITOOLIGOSACCHARIDE ELICITOR ; CULTURED RICE CELLS ; SYSTEMIC ACQUIRED-RESISTANCE ; PHENYLALANINE AMMONIA-LYASE ; DISEASE RESISTANCE ; DEFENSE RESPONSES ; PLASMA-MEMBRANE ; SIGNAL-TRANSDUCTION ; CHITIN FRAGMENTS
英文摘要Oligochitosan induces defense responses to pathogenic microbes in a wide variety of plants by acting as an elicitor. In the present study, mRNA differential display was used to investigate oligochitosan-induced transcriptional activation of defense-related genes. Accordingly, a novel Ser/Thr protein kinase gene was isolated and designated as oligochitosan-induced protein kinase (oipk). Molecular cloning showed that oipk contains six introns interrupted by seven exons. The open reading frame (ORF) of the gene is 1848 bp, which encodes a putative protein of 615 amino acids with the predicted molecular mass of 70.96 kDa and a pI of 6.32. A plant oipk antisense expression vector was constructed and transformed into tobacco by Agrobacterium tumefaciens. Decreased phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activity and decreased resistance to tobacco mosaic virus (TMV) were observed in transgenic tobacco. RT-PCR analysis revealed that oipk was expressed at high levels after oligochitosan induction in wild-type tobacco, but not in transgenic tobacco. These results indicated that oipk is involved in the signal pathway of oligochitosan-induced resistance in tobacco. (c) 2006 Elsevier Masson SAS. All rights reserved.
语种英语
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WOS记录号WOS:000242710500011
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被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98331
专题中国科学院大连化学物理研究所
通讯作者Feng B(冯斌)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Liaoning Normal Univ, Coll Life Sci, Dalian 116029, Peoples R China
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Feng, B.,Chen, Y.,Zhao, C.,et al. Isolation of a novel Ser/Thr protein kinase gene from oligochitosan-induced tobacco and its role in resistance against tobacco mosaic virus[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2006,44(10):596-603.
APA Feng, B..,Chen, Y..,Zhao, C..,Zhao, X..,Bai, X..,...&冯斌.(2006).Isolation of a novel Ser/Thr protein kinase gene from oligochitosan-induced tobacco and its role in resistance against tobacco mosaic virus.PLANT PHYSIOLOGY AND BIOCHEMISTRY,44(10),596-603.
MLA Feng, B.,et al."Isolation of a novel Ser/Thr protein kinase gene from oligochitosan-induced tobacco and its role in resistance against tobacco mosaic virus".PLANT PHYSIOLOGY AND BIOCHEMISTRY 44.10(2006):596-603.
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