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Advances in chromatographic techniques and methods in shotgun proteome analysis
Ye, Mingliang; Jiang, Xiaogang; Feng, Shun; Tian, Ruijun; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
KeywordAutomated Proteome-analysis System Multidimensional Separation One-dimensional Separation Separation Technique Shotgun Proteomics
Source PublicationTRAC-TRENDS IN ANALYTICAL CHEMISTRY
2007
ISSN0165-9936
DOI10.1016/j.trac.2006.10.012
Volume26Issue:1Pages:80-84
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordTANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; FREE-FLOW ELECTROPHORESIS ; HUMAN PLASMA PROTEOME ; CODED AFFINITY TAGS ; MULTIDIMENSIONAL CHROMATOGRAPHY ; CAPILLARY COLUMNS ; NANOELECTROSPRAY IONIZATION ; IDENTIFICATION TECHNOLOGY ; MONOLITHIC CAPILLARY
AbstractShotgun proteomics is a high-throughput approach to proteome analysis whereby the protein mixture is digested and the peptides generated are separated by capillary liquid chromatography and sequenced by tandem mass spectrometry (MS). Due to the huge number of peptide species, separation prior to MS analysis plays an important role in shotgun proteomics. Overall sensitivity, dynamic range, throughput and general effectiveness of shotgun proteomic analysis largely depend on how well the peptide mixture is separated. In recent years, new separation techniques have been applied successfully to proteome analysis and have dramatically improved protein identification. We briefly review the recent development of chromatographic techniques and methods in shotgun proteome analysis, including the following three aspects: one-dimensional separation; multidimensional separation; and, automated proteome-analysis systems. (c) 2006 Elsevier Ltd. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000244120400021
Citation statistics
Cited Times:40[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/98433
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Ye, Mingliang,Jiang, Xiaogang,Feng, Shun,et al. Advances in chromatographic techniques and methods in shotgun proteome analysis[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2007,26(1):80-84.
APA Ye, Mingliang.,Jiang, Xiaogang.,Feng, Shun.,Tian, Ruijun.,Zou, Hanfa.,...&邹汉法.(2007).Advances in chromatographic techniques and methods in shotgun proteome analysis.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,26(1),80-84.
MLA Ye, Mingliang,et al."Advances in chromatographic techniques and methods in shotgun proteome analysis".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 26.1(2007):80-84.
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