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Advances in chromatographic techniques and methods in shotgun proteome analysis
Ye, Mingliang; Jiang, Xiaogang; Feng, Shun; Tian, Ruijun; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Automated Proteome-analysis System Multidimensional Separation One-dimensional Separation Separation Technique Shotgun Proteomics
刊名TRAC-TRENDS IN ANALYTICAL CHEMISTRY
2007
ISSN0165-9936
DOI10.1016/j.trac.2006.10.012
26期:1页:80-84
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; FREE-FLOW ELECTROPHORESIS ; HUMAN PLASMA PROTEOME ; CODED AFFINITY TAGS ; MULTIDIMENSIONAL CHROMATOGRAPHY ; CAPILLARY COLUMNS ; NANOELECTROSPRAY IONIZATION ; IDENTIFICATION TECHNOLOGY ; MONOLITHIC CAPILLARY
英文摘要Shotgun proteomics is a high-throughput approach to proteome analysis whereby the protein mixture is digested and the peptides generated are separated by capillary liquid chromatography and sequenced by tandem mass spectrometry (MS). Due to the huge number of peptide species, separation prior to MS analysis plays an important role in shotgun proteomics. Overall sensitivity, dynamic range, throughput and general effectiveness of shotgun proteomic analysis largely depend on how well the peptide mixture is separated. In recent years, new separation techniques have been applied successfully to proteome analysis and have dramatically improved protein identification. We briefly review the recent development of chromatographic techniques and methods in shotgun proteome analysis, including the following three aspects: one-dimensional separation; multidimensional separation; and, automated proteome-analysis systems. (c) 2006 Elsevier Ltd. All rights reserved.
语种英语
原文出处查看原文
WOS记录号WOS:000244120400021
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98433
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Ye, Mingliang,Jiang, Xiaogang,Feng, Shun,et al. Advances in chromatographic techniques and methods in shotgun proteome analysis[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2007,26(1):80-84.
APA Ye, Mingliang.,Jiang, Xiaogang.,Feng, Shun.,Tian, Ruijun.,Zou, Hanfa.,...&邹汉法.(2007).Advances in chromatographic techniques and methods in shotgun proteome analysis.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,26(1),80-84.
MLA Ye, Mingliang,et al."Advances in chromatographic techniques and methods in shotgun proteome analysis".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 26.1(2007):80-84.
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