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题名: Analysis of oxidized multi-walled carbon nanotubes in single K562 cells by capillary electrophoresis with laser-induced fluorescence
作者: Xiao, Hua;  Yang, Lisong;  Zou, Hanfa;  Yang, Ling;  Le, X. Chris
通讯作者: 邹汉法
关键词: multi-walled carbon nanotubes ;  single-cell analysis ;  multidrug resistance ;  capillary electrophoresis ;  laser-induced fluorescence
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2007
DOI: 10.1007/s00216-006-0654-4
卷: 387, 期:1, 页:119-126
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Short oxidized multi-walled carbon nanotubes (CNT) were derivatized with fluorescein isothiocyanate (FITC). Capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF) was then used to separate and detect the fluorescently labeled carbon-nanotube probes (CNTP) in multidrug-resistant cells (K562A) and the parent cells (K562S). Greater expression of P-glycoprotein in K562A cells than in K562S cells was confirmed by use of anti-P-glycoprotein antibody and flow-cytometric analysis. Analyses of CNTP in both cell lines using both CE-LIF and flow cytometry showed that CNTP could traverse the cellular membrane without being pumped out by P-glycoprotein. The CNTP distributed in both cell lines was analyzed at the single cell level and the results were compared with those from analysis of ten cells and of the lysate from bulk cells. The results revealed the CE-LIF method could be used for quantitative analysis of CNT in single cells in studies of drug delivery and multidrug resistance.
关键词[WOS]: DRUG-DELIVERY ;  P-GLYCOPROTEIN ;  TRANSPORTERS ;  RESISTANCE ;  CANCER ;  PROTEINS ;  FIELD ;  DNA
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000242968000021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98445
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resources Discovery, Dalian 116023, Peoples R China
3.Univ Alberta, Fac Med, Dept Publ Hlth Sci, Edmonton, AB T6G 2G3, Canada

Recommended Citation:
Xiao, Hua,Yang, Lisong,Zou, Hanfa,et al. Analysis of oxidized multi-walled carbon nanotubes in single K562 cells by capillary electrophoresis with laser-induced fluorescence[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2007,387(1):119-126.
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