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Analysis of oxidized multi-walled carbon nanotubes in single K562 cells by capillary electrophoresis with laser-induced fluorescence
Xiao, Hua; Yang, Lisong; Zou, Hanfa; Yang, Ling; Le, X. Chris; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Multi-walled Carbon Nanotubes Single-cell Analysis Multidrug Resistance Capillary Electrophoresis Laser-induced Fluorescence
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2007
ISSN1618-2642
DOI10.1007/s00216-006-0654-4
387期:1页:119-126
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]DRUG-DELIVERY ; P-GLYCOPROTEIN ; TRANSPORTERS ; RESISTANCE ; CANCER ; PROTEINS ; FIELD ; DNA
英文摘要Short oxidized multi-walled carbon nanotubes (CNT) were derivatized with fluorescein isothiocyanate (FITC). Capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF) was then used to separate and detect the fluorescently labeled carbon-nanotube probes (CNTP) in multidrug-resistant cells (K562A) and the parent cells (K562S). Greater expression of P-glycoprotein in K562A cells than in K562S cells was confirmed by use of anti-P-glycoprotein antibody and flow-cytometric analysis. Analyses of CNTP in both cell lines using both CE-LIF and flow cytometry showed that CNTP could traverse the cellular membrane without being pumped out by P-glycoprotein. The CNTP distributed in both cell lines was analyzed at the single cell level and the results were compared with those from analysis of ten cells and of the lysate from bulk cells. The results revealed the CE-LIF method could be used for quantitative analysis of CNT in single cells in studies of drug delivery and multidrug resistance.
语种英语
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WOS记录号WOS:000242968000021
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98445
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resources Discovery, Dalian 116023, Peoples R China
3.Univ Alberta, Fac Med, Dept Publ Hlth Sci, Edmonton, AB T6G 2G3, Canada
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GB/T 7714
Xiao, Hua,Yang, Lisong,Zou, Hanfa,et al. Analysis of oxidized multi-walled carbon nanotubes in single K562 cells by capillary electrophoresis with laser-induced fluorescence[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2007,387(1):119-126.
APA Xiao, Hua.,Yang, Lisong.,Zou, Hanfa.,Yang, Ling.,Le, X. Chris.,...&邹汉法.(2007).Analysis of oxidized multi-walled carbon nanotubes in single K562 cells by capillary electrophoresis with laser-induced fluorescence.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,387(1),119-126.
MLA Xiao, Hua,et al."Analysis of oxidized multi-walled carbon nanotubes in single K562 cells by capillary electrophoresis with laser-induced fluorescence".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 387.1(2007):119-126.
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