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题名: Comprehensive peptidome analysis of mouse livers by size exclusion chromatography prefractionation and NanoLC-MS/MS identification
作者: Hu, Lianghai;  Li, Xin;  Jiang, Xinning;  Zhou, Houjiang;  Jiang, Xiaogang;  Kong, Liang;  Ye, Mingliang;  Zou, Hanfa
通讯作者: 邹汉法
关键词: peptidomics ;  proteomics ;  mouse liver ;  size exclusion chromatography ;  mass spectrometry
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2007-02-01
DOI: 10.1021/pr060469e
卷: 6, 期:2, 页:801-808
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Peptidome analysis has received increasing attention in recent years. Cancer diagnosis by serum peptidome has also been reported by peptides' profiling for discovery of peptide biomarkers. Tissue, which may have a higher biomarker concentration than blood, has not been investigated extensively by means of peptidome analysis. Here, a method for the peptidome analysis of mouse liver was developed by the combination of size exclusion chromatography (SEC) prefractionation with nano-liquid chromatography-tamdem mass spectrometry (nanoLC-MS/MS) analysis. The extracted peptides from mouse liver were separated according to their molecular weight using a size exclusion column. MALDI-TOF MS was used to characterize the molecular weight distribution of the peptides in fractions eluted from the SEC column. The low molecular weight (LMW) (MW < 3000 Da) peptides in the collected fractions were directly analyzed by LC-MS/MS which resulted in the identification of 1181 unique peptides (from 371 proteins). The high molecular weight (HMW) (MW > 3000 Da) peptides in the early two fractions from the SEC column were first digested with trypsin, and the resulted digests were then analyzed by LC-MS/MS, which led to the identification of 123 and 127 progenitor proteins of the HMW peptides in fractions 1 and 2, respectively. Analysis of the peptides' cleavage sites showed that the peptides are cleaved in regulation, which may reflect the protease activity and distribution in body, and also represent the biological state of the tissue and provide a fresh source for biomarker discovery.
关键词[WOS]: SPECTROMETRY-BASED PROTEOMICS ;  MASS-SPECTROMETRY ;  SERUM PEPTIDOME ;  ENDOGENOUS PEPTIDES ;  OVARIAN-CANCER ;  BIOMARKERS ;  PATTERNS ;  COMPLEX ;  NEUROPEPTIDES ;  TECHNOLOGY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000243927900035
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98457
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Hu, Lianghai,Li, Xin,Jiang, Xinning,et al. Comprehensive peptidome analysis of mouse livers by size exclusion chromatography prefractionation and NanoLC-MS/MS identification[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(2):801-808.
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