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Comprehensive peptidome analysis of mouse livers by size exclusion chromatography prefractionation and NanoLC-MS/MS identification
Hu, Lianghai; Li, Xin; Jiang, Xinning; Zhou, Houjiang; Jiang, Xiaogang; Kong, Liang; Ye, Mingliang; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
KeywordPeptidomics Proteomics Mouse Liver Size Exclusion Chromatography Mass Spectrometry
Source PublicationJOURNAL OF PROTEOME RESEARCH
2007-02-01
ISSN1535-3893
DOI10.1021/pr060469e
Volume6Issue:2Pages:801-808
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordSPECTROMETRY-BASED PROTEOMICS ; MASS-SPECTROMETRY ; SERUM PEPTIDOME ; ENDOGENOUS PEPTIDES ; OVARIAN-CANCER ; BIOMARKERS ; PATTERNS ; COMPLEX ; NEUROPEPTIDES ; TECHNOLOGY
AbstractPeptidome analysis has received increasing attention in recent years. Cancer diagnosis by serum peptidome has also been reported by peptides' profiling for discovery of peptide biomarkers. Tissue, which may have a higher biomarker concentration than blood, has not been investigated extensively by means of peptidome analysis. Here, a method for the peptidome analysis of mouse liver was developed by the combination of size exclusion chromatography (SEC) prefractionation with nano-liquid chromatography-tamdem mass spectrometry (nanoLC-MS/MS) analysis. The extracted peptides from mouse liver were separated according to their molecular weight using a size exclusion column. MALDI-TOF MS was used to characterize the molecular weight distribution of the peptides in fractions eluted from the SEC column. The low molecular weight (LMW) (MW < 3000 Da) peptides in the collected fractions were directly analyzed by LC-MS/MS which resulted in the identification of 1181 unique peptides (from 371 proteins). The high molecular weight (HMW) (MW > 3000 Da) peptides in the early two fractions from the SEC column were first digested with trypsin, and the resulted digests were then analyzed by LC-MS/MS, which led to the identification of 123 and 127 progenitor proteins of the HMW peptides in fractions 1 and 2, respectively. Analysis of the peptides' cleavage sites showed that the peptides are cleaved in regulation, which may reflect the protease activity and distribution in body, and also represent the biological state of the tissue and provide a fresh source for biomarker discovery.
Language英语
URL查看原文
WOS IDWOS:000243927900035
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/98457
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Hu, Lianghai,Li, Xin,Jiang, Xinning,et al. Comprehensive peptidome analysis of mouse livers by size exclusion chromatography prefractionation and NanoLC-MS/MS identification[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(2):801-808.
APA Hu, Lianghai.,Li, Xin.,Jiang, Xinning.,Zhou, Houjiang.,Jiang, Xiaogang.,...&邹汉法.(2007).Comprehensive peptidome analysis of mouse livers by size exclusion chromatography prefractionation and NanoLC-MS/MS identification.JOURNAL OF PROTEOME RESEARCH,6(2),801-808.
MLA Hu, Lianghai,et al."Comprehensive peptidome analysis of mouse livers by size exclusion chromatography prefractionation and NanoLC-MS/MS identification".JOURNAL OF PROTEOME RESEARCH 6.2(2007):801-808.
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