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题名: Probing the role of microenvironment for microencapsulated Sacchromyces cerevisiae under osmotic stress
作者: Sun, Zhi-Jie;  Lv, Guo-Jun;  Li, Shuang-Yue;  Xie, Yu-Bing;  Yu, Wei-Ting;  Wang, W.;  Ma, Xiao-Jun
通讯作者: 马小军
关键词: yeast ;  viability ;  alginate microcapsule ;  microenvironment ;  osmotic stress
刊名: JOURNAL OF BIOTECHNOLOGY
发表日期: 2007-01-30
DOI: 10.1016/j.jbiotec.2006.09.001
卷: 128, 期:1, 页:150-161
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Cell encapsulation opens a new avenue to the oral delivery of genetically engineered microorganism for therapeutic purpose. Osmotic stress is one of the universal chemical stress factors in the application of microencapsulation technology. In order to understand the effect and mechanism of the encapsulated microenvironment on protecting cells from hyper-osmotic stress, yeast cells of Saccharomyces cerevisiae Y800 were encapsulated in calcium alginate micro-gel beads (MB), alginate-chitosan-algin ate (ACA) solid core microcapsules (SCM), and ACA liquid core microcapsules (LCM), respectively. The stress-induced intracellular components and enzyme activity including trehalose, glycerol and super oxide dismutase (SOD) were measured. Free cell culture was used as control. The survival of encapsulated cells and the cells released from MB, SCM and LCM after osmotic shock induced by NaCl solution (1, 2 and 3 M) was evaluated. An analysis method was established to probe the effect of encapsulated microenvironment on the cell tolerance to osmotic stress. The results showed that LCM gave rise to the highest level of intracellular trehalose and glycerol, and SOD activity, as well as the highest survival rate of encapsulated cells or cells released from microcapsule. It was demonstrated that LCM was able to induce the highest stress response and stress tolerance of cells, which was adapted during culture, while SCM failed. The theoretical analysis revealed that it was the liquid alginate matrix in microcapsule that played a central role in domesticating the cells to adapt to hyper-osmotic stress. This finding provides a very useful guideline to cell encapsulation. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: ENGINEERED ESCHERICHIA-COLI ;  SACCHAROMYCES-CEREVISIAE ;  ALGINATE MICROCAPSULES ;  INTESTINAL DELIVERY ;  LACTOCOCCUS-LACTIS ;  ARTIFICIAL CELLS ;  DH5 CELLS ;  IN-VITRO ;  YEAST ;  TOLERANCE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000243733700015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98465
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Sci & Technol, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Sun, Zhi-Jie,Lv, Guo-Jun,Li, Shuang-Yue,et al. Probing the role of microenvironment for microencapsulated Sacchromyces cerevisiae under osmotic stress[J]. JOURNAL OF BIOTECHNOLOGY,2007,128(1):150-161.
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