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Microcalorimetric study on the growth and metabolism of microencapsulated microbial cell culture
Ma, J.; Qi, W. T.; Yang, L. N.; Yu, W. T.; Xie, Y. B.; Wang, W.; Ma, X. J.; Xu, F.; Sun, L. X.; Ma XJ(马小军); Ma XJ(马小军)
KeywordMicrocalorimetry Microencapsulation Microbial Growth And Metabolism
Source PublicationJOURNAL OF MICROBIOLOGICAL METHODS
2007
DOI10.1016/j.mimet.2006.07.007
Volume68Issue:1Pages:172-177
Indexed BySCI
SubtypeArticle
Department18,15
Funding Project1802,1504
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods ; Microbiology
WOS Research AreaBiochemistry & Molecular Biology ; Microbiology
WOS KeywordARTIFICIAL CELLS ; MAMMALIAN-CELLS ; ALGINATE BEADS ; ENCAPSULATION ; MICROCAPSULE ; DELIVERY
AbstractThe rate of heat output is one of the suitable measurements of metabolic activity of the organism or its parts, down to the cellular or even the sub-cellular levels. In this paper, microcalorimetry was first applied to study the metabolic activity of microbial in both alginate-polylysine-alginate and alginate-chitosan-alginate microencapsulated cultures as well as in free non-encapsulated culture. The organisms used for the measurements were Escherichia coli and Saccharomyces cerevisiae. As a result of this work, it was found that, despite E. coli cell in free non-encapsulated culture has the highest metabolic rate due to the highest value of heat output, the proliferation of the cells terminates quickly with a lowest biomass formed. And we found also an obviously longer stationary phase in microencapsulated culture. As far as S. cerevisiae was concerned, it was found that there was also the highest value of heat output in free non-encapsulated culture, but the cell density was lower than that in microencapsulated culture. On account of the microcalorimetric and metabolic measurements, it can be concluded that more substrate can be used to convert to biomass in microencapsulated culture which means a higher biomass yield existed. (c) 2006 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000244012300022
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/98467
Collection中国科学院大连化学物理研究所
Corresponding AuthorMa XJ(马小军); Ma XJ(马小军)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Thermochem Lab, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Ma, J.,Qi, W. T.,Yang, L. N.,et al. Microcalorimetric study on the growth and metabolism of microencapsulated microbial cell culture[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2007,68(1):172-177.
APA Ma, J..,Qi, W. T..,Yang, L. N..,Yu, W. T..,Xie, Y. B..,...&马小军.(2007).Microcalorimetric study on the growth and metabolism of microencapsulated microbial cell culture.JOURNAL OF MICROBIOLOGICAL METHODS,68(1),172-177.
MLA Ma, J.,et al."Microcalorimetric study on the growth and metabolism of microencapsulated microbial cell culture".JOURNAL OF MICROBIOLOGICAL METHODS 68.1(2007):172-177.
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