中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Adipogenesis of murine embryonic stem cells in a three-dimensional culture system using electrospun polymer scaffolds
作者: Kang, Xihai;  Xie, Yubing;  Powell, Heather M.;  Lee, L. James;  Belury, Martha A.;  Lannutti, John J.;  Kniss, Douglas A.
通讯作者: Douglas A. Kniss
关键词: adipose tissue engineering ;  stem cell ;  electrospinning ;  polycaprolactone ;  three-dimensional cell culture ;  morphogenesis
刊名: BIOMATERIALS
发表日期: 2007
DOI: 10.1016/j.biomaterials.2006.08.052
卷: 28, 期:3, 页:450-458
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 7;2
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Materials Science, Biomaterials
研究领域[WOS]: Engineering ;  Materials Science
英文摘要: A mechanistic understanding of adipose tissue differentiation is critical for the treatment and prevention of obesity and type 2 diabetes. Conventional in vitro models of adipogenesis are preadipocytes or freshly isolated adipocytes grown in two-dimensional (2D) cultures. Optimal results using in vitro tissue culture models can be expected only when adipocyte models closely resemble adipose tissue in vivo. Thus the design of an in vitro three-dimensional (3D) model which faithfully mimics the in vivo environment is needed to effectively study adipogenesis. Pluripotent embryonic stem (ES) cells are a self-renewing cell type that can readily be differentiated into adipocytes. In this study, a 3D culture system was developed to mimic the geometry of adipose tissue in vivo. Murine ES cells were seeded into electrospun polycaprolactone scaffolds and differentiated into adipocytes in situ by hormone induction as demonstrated using a battery of gene and protein expression markers along with the accumulation of neutral lipid droplets. Insulin-responsive Akt phosphorylation, and beta-adrenergic stimulation of cyclic AMP synthesis were demonstrated in ES cell-derived adipocytes. Morphologically, ES cell-derived adipocytes resembled native fat cells by scanning electron and phase contrast microscopy. This tissue engineered ES cell-matrix model has potential uses in drug screening and other therapeutic developments. (c) 2006 Elsevier Ltd. All rights reserved.
关键词[WOS]: FIBROBLAST-GROWTH-FACTOR ;  ADIPOSE-TISSUE ;  IN-VITRO ;  ADIPOCYTE DIFFERENTIATION ;  VIVO ;  EXPRESSION ;  BIOLOGY ;  MODEL ;  MORPHOGENESIS ;  ORGANIZATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000242960900009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98469
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Ohio State Univ, Coll Med, Dept Obstet & Gynecol, Lab Perinatal Res, Columbus, OH 43210 USA
2.Ohio State Univ, Nanoscale Sci & Engn Ctr Affordable Nanoengn Poly, Columbus, OH 43210 USA
3.Ohio State Univ, Dept Biomed Engn, Columbus, OH 43210 USA
4.Ohio State Univ, Dept Mat Sci & Engn, Columbus, OH 43210 USA
5.Ohio State Univ, Dept Chem & Biomol Engn, Columbus, OH 43210 USA
6.Ohio State Univ, Dept Human Nutr, Columbus, OH 43210 USA
7.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116021, Peoples R China

Recommended Citation:
Kang, Xihai,Xie, Yubing,Powell, Heather M.,et al. Adipogenesis of murine embryonic stem cells in a three-dimensional culture system using electrospun polymer scaffolds[J]. BIOMATERIALS,2007,28(3):450-458.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Kang, Xihai]'s Articles
 [Xie, Yubing]'s Articles
 [Powell, Heather M.]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Kang, Xihai]‘s Articles
 [Xie, Yubing]‘s Articles
 [Powell, Heather M.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace