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题名: Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome analysis by using a strong cation exchange trap column
作者: Jiang, Xiaogang;  Feng, Shun;  Tian, Ruijun;  Han, Guanghui;  Jiang, Xinning;  Ye, Mingliang;  Zou, Hanfa
通讯作者: 邹汉法
关键词: automation ;  mu LC-MS/MS ;  sample injection ;  SCX trap column
刊名: PROTEOMICS
发表日期: 2007-02-01
DOI: 10.1002/pmic.200600661
卷: 7, 期:4, 页:528-539
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: An approach was developed to automate sample introduction for nanoflow LC-MS/MS (mu LC-MS/ MS) analysis using a strong cation exchange (SCX) trap column. The system consisted of a 100 mu m id x 2 cm SCX trap column and a 75 mu m id x 12 cm C18 RP analytical column. During the sample loading step, the flow passing through the SCX trap column was directed to waste for loading a large volume of sample at high flow rate. Then the peptides bound on the SCX trap column were eluted onto the RP analytical column by a high salt buffer followed by RP chromatographic separation of the peptides at nanoliter flow rate. It was observed that higher performance of separation could be achieved with the system using SCX trap column than with the system using C18 trap column. The high proteomic coverage using this approach was demonstrated in the analysis of tryptic digest of BSA and yeast cell lysate. in addition, this system was also applied to two-dimensional separation of tryptic digest of human hepatocellular carcinoma cell line SMMC-7721 for large scale proteome analysis. This system was fully automated and required minimum changes on current mu LC-MS/MS system. This system represented a promising platform for routine proteome analysis.
关键词[WOS]: MULTIDIMENSIONAL CHROMATOGRAPHY ;  IDENTIFICATION TECHNOLOGY ;  POLYACRYLAMIDE-GEL ;  YEAST PROTEOME ;  MS SYSTEM ;  ELECTROSPRAY ;  IONIZATION ;  CAPILLARY ;  PROTEINS ;  PEPTIDE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000244807500006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98477
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Suzhou Univ, Sch Med, Suzhou 215006, Jiangsu, Peoples R China

Recommended Citation:
Jiang, Xiaogang,Feng, Shun,Tian, Ruijun,et al. Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome analysis by using a strong cation exchange trap column[J]. PROTEOMICS,2007,7(4):528-539.
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