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Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues
Jiang, Xiaogang; Jiang, Xinning; Feng, Shun; Tian, Ruijun; Ye, Mingliang; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
KeywordFormalin-fixed Tissue Protein Extraction Shotgun Proteome Tandem Mass Spectrometry
Source PublicationJOURNAL OF PROTEOME RESEARCH
2007-03-01
ISSN1535-3893
DOI10.1021/pr0605318
Volume6Issue:3Pages:1038-1047
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordDIFFERENCE GEL-ELECTROPHORESIS ; TANDEM MASS-SPECTROMETRY ; CANDIDATE CANCER BIOMARKERS ; PARAFFIN-EMBEDDED TISSUE ; IONIZATION EMITTER ; YEAST PROTEOME ; IDENTIFICATION ; MS/MS ; IMMUNOHISTOCHEMISTRY ; SEPARATION
AbstractThere are vast archives of formalin-fixed tissues spanning many conceivable conditions such as different diseases, time courses, and different treatment and allowing acquisition of the necessary numbers of samples to carry out biomarker discovery study. However, the conventional protein analysis approach is not applicable for the analysis of proteins in the formalin-fixed tissue because the formalin fixation process resulted in the cross-linking of proteins, and thus, intact proteins cannot be efficiently extracted. In this study, several protocols were investigated to extract proteins from formalin-fixed mouse liver tissue for shotgun proteome analysis. It was found that incubation of tissue in a lysis buffer containing 6 M guanidine hydrochloride at high temperature led to the highest protein yield and the largest number of proteins identified. The peptides and proteins identified from formalin-fixed tissue were first comprehensively compared with those identified from frozen-fresh tissue. It was found that a majority of peptides identified from fixed tissue were unmodified and proteome coverage for the analysis of fixed tissue was not obviously compromised by the formalin fixation process. Valuable proteome information could be obtained by shotgun proteome analysis of formalin-fixed tissue, which presents a new approach for disease biomarker discovery.
Language英语
URL查看原文
WOS IDWOS:000244638400012
Citation statistics
Cited Times:64[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/98493
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog B&A Ctr, Dalian 116023, Peoples R China
2.Suzhou Univ, Sch Med, Suzhou 215007, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Xiaogang,Jiang, Xinning,Feng, Shun,et al. Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(3):1038-1047.
APA Jiang, Xiaogang.,Jiang, Xinning.,Feng, Shun.,Tian, Ruijun.,Ye, Mingliang.,...&邹汉法.(2007).Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues.JOURNAL OF PROTEOME RESEARCH,6(3),1038-1047.
MLA Jiang, Xiaogang,et al."Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues".JOURNAL OF PROTEOME RESEARCH 6.3(2007):1038-1047.
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