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Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues
Jiang, Xiaogang; Jiang, Xinning; Feng, Shun; Tian, Ruijun; Ye, Mingliang; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Formalin-fixed Tissue Protein Extraction Shotgun Proteome Tandem Mass Spectrometry
刊名JOURNAL OF PROTEOME RESEARCH
2007-03-01
ISSN1535-3893
DOI10.1021/pr0605318
6期:3页:1038-1047
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]DIFFERENCE GEL-ELECTROPHORESIS ; TANDEM MASS-SPECTROMETRY ; CANDIDATE CANCER BIOMARKERS ; PARAFFIN-EMBEDDED TISSUE ; IONIZATION EMITTER ; YEAST PROTEOME ; IDENTIFICATION ; MS/MS ; IMMUNOHISTOCHEMISTRY ; SEPARATION
英文摘要There are vast archives of formalin-fixed tissues spanning many conceivable conditions such as different diseases, time courses, and different treatment and allowing acquisition of the necessary numbers of samples to carry out biomarker discovery study. However, the conventional protein analysis approach is not applicable for the analysis of proteins in the formalin-fixed tissue because the formalin fixation process resulted in the cross-linking of proteins, and thus, intact proteins cannot be efficiently extracted. In this study, several protocols were investigated to extract proteins from formalin-fixed mouse liver tissue for shotgun proteome analysis. It was found that incubation of tissue in a lysis buffer containing 6 M guanidine hydrochloride at high temperature led to the highest protein yield and the largest number of proteins identified. The peptides and proteins identified from formalin-fixed tissue were first comprehensively compared with those identified from frozen-fresh tissue. It was found that a majority of peptides identified from fixed tissue were unmodified and proteome coverage for the analysis of fixed tissue was not obviously compromised by the formalin fixation process. Valuable proteome information could be obtained by shotgun proteome analysis of formalin-fixed tissue, which presents a new approach for disease biomarker discovery.
语种英语
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WOS记录号WOS:000244638400012
引用统计
被引频次:63[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98493
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog B&A Ctr, Dalian 116023, Peoples R China
2.Suzhou Univ, Sch Med, Suzhou 215007, Jiangsu, Peoples R China
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GB/T 7714
Jiang, Xiaogang,Jiang, Xinning,Feng, Shun,et al. Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(3):1038-1047.
APA Jiang, Xiaogang.,Jiang, Xinning.,Feng, Shun.,Tian, Ruijun.,Ye, Mingliang.,...&邹汉法.(2007).Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues.JOURNAL OF PROTEOME RESEARCH,6(3),1038-1047.
MLA Jiang, Xiaogang,et al."Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues".JOURNAL OF PROTEOME RESEARCH 6.3(2007):1038-1047.
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