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题名: Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues
作者: Jiang, Xiaogang;  Jiang, Xinning;  Feng, Shun;  Tian, Ruijun;  Ye, Mingliang;  Zou, Hanfa
通讯作者: 邹汉法
关键词: formalin-fixed tissue ;  protein extraction ;  shotgun proteome ;  tandem mass spectrometry
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2007-03-01
DOI: 10.1021/pr0605318
卷: 6, 期:3, 页:1038-1047
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: There are vast archives of formalin-fixed tissues spanning many conceivable conditions such as different diseases, time courses, and different treatment and allowing acquisition of the necessary numbers of samples to carry out biomarker discovery study. However, the conventional protein analysis approach is not applicable for the analysis of proteins in the formalin-fixed tissue because the formalin fixation process resulted in the cross-linking of proteins, and thus, intact proteins cannot be efficiently extracted. In this study, several protocols were investigated to extract proteins from formalin-fixed mouse liver tissue for shotgun proteome analysis. It was found that incubation of tissue in a lysis buffer containing 6 M guanidine hydrochloride at high temperature led to the highest protein yield and the largest number of proteins identified. The peptides and proteins identified from formalin-fixed tissue were first comprehensively compared with those identified from frozen-fresh tissue. It was found that a majority of peptides identified from fixed tissue were unmodified and proteome coverage for the analysis of fixed tissue was not obviously compromised by the formalin fixation process. Valuable proteome information could be obtained by shotgun proteome analysis of formalin-fixed tissue, which presents a new approach for disease biomarker discovery.
关键词[WOS]: DIFFERENCE GEL-ELECTROPHORESIS ;  TANDEM MASS-SPECTROMETRY ;  CANDIDATE CANCER BIOMARKERS ;  PARAFFIN-EMBEDDED TISSUE ;  IONIZATION EMITTER ;  YEAST PROTEOME ;  IDENTIFICATION ;  MS/MS ;  IMMUNOHISTOCHEMISTRY ;  SEPARATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000244638400012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98493
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog B&A Ctr, Dalian 116023, Peoples R China
2.Suzhou Univ, Sch Med, Suzhou 215007, Jiangsu, Peoples R China

Recommended Citation:
Jiang, Xiaogang,Jiang, Xinning,Feng, Shun,et al. Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(3):1038-1047.
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