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题名: Preparation of a biochip on porous silicon and application for label-free detection of small molecule-protein interactions
作者: Hu, Ligang;  Xu, Songyun;  Pan, Chensong;  Zou, Hanfa;  Jiang, Guibin
通讯作者: 江桂斌 ;  邹汉法
刊名: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表日期: 2007
DOI: 10.1002/rcm.2944
卷: 21, 期:7, 页:1277-1281
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 2;2
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Analytical ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Spectroscopy
英文摘要: A new approach for the preparation of a biochip on porous silicon and the application of the biochip for detection of small molecule-protein interactions with desorption/ionization on porous silicon (DIOS) was demonstrated. The galvanostatically etched porous silicon substrates were chemically modified firstly to yield carboxylic acid terminated surfaces, and then the protein was covalently attached to the surface through amide bonding. By applying a solution of candidate chemicals to the surface and a subsequent wash step, the masses of captured compounds could be analyzed by DIOS. DIOS has advantages of being a direct detection tool compared to the classic florescence or chemiluminescence methods, because the process of labeling molecules employed in the florescence or chemiluminescence methods can sometimes alert the properties of the labeled molecule. The recognition between proteins and their binding partners is efficient and selective. A good tolerance to disturbance and high enrichment factor of the biochip to the analytes was observed. As an on-chip-based approach, the demonstrated method has a potential to perform in a high-throughput format. Copyright (c) 2007 John Wiley & Sons, Ltd.
关键词[WOS]: DESORPTION/IONIZATION MASS-SPECTROMETRY ;  ENZYME IMMOBILIZATION ;  BINDING ;  FABRICATION ;  BIOSENSOR ;  SURFACES ;  PLATFORM ;  MATRIX ;  ASSAY ;  RAT
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000245210900022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98495
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Hu, Ligang,Xu, Songyun,Pan, Chensong,et al. Preparation of a biochip on porous silicon and application for label-free detection of small molecule-protein interactions[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2007,21(7):1277-1281.
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