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Preparation of a biochip on porous silicon and application for label-free detection of small molecule-protein interactions
Hu, Ligang; Xu, Songyun; Pan, Chensong; Zou, Hanfa; Jiang, Guibin; Jiang GB(江桂斌); Zou HF(邹汉法); Jiang GB(江桂斌); Zou HF(邹汉法)
Source PublicationRAPID COMMUNICATIONS IN MASS SPECTROMETRY
2007
ISSN0951-4198
DOI10.1002/rcm.2944
Volume21Issue:7Pages:1277-1281
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank2;2
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Analytical ; Spectroscopy
WOS Research AreaChemistry ; Spectroscopy
WOS KeywordDESORPTION/IONIZATION MASS-SPECTROMETRY ; ENZYME IMMOBILIZATION ; BINDING ; FABRICATION ; BIOSENSOR ; SURFACES ; PLATFORM ; MATRIX ; ASSAY ; RAT
AbstractA new approach for the preparation of a biochip on porous silicon and the application of the biochip for detection of small molecule-protein interactions with desorption/ionization on porous silicon (DIOS) was demonstrated. The galvanostatically etched porous silicon substrates were chemically modified firstly to yield carboxylic acid terminated surfaces, and then the protein was covalently attached to the surface through amide bonding. By applying a solution of candidate chemicals to the surface and a subsequent wash step, the masses of captured compounds could be analyzed by DIOS. DIOS has advantages of being a direct detection tool compared to the classic florescence or chemiluminescence methods, because the process of labeling molecules employed in the florescence or chemiluminescence methods can sometimes alert the properties of the labeled molecule. The recognition between proteins and their binding partners is efficient and selective. A good tolerance to disturbance and high enrichment factor of the biochip to the analytes was observed. As an on-chip-based approach, the demonstrated method has a potential to perform in a high-throughput format. Copyright (c) 2007 John Wiley & Sons, Ltd.
Language英语
URL查看原文
WOS IDWOS:000245210900022
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/98495
Collection中国科学院大连化学物理研究所
Corresponding AuthorJiang GB(江桂斌); Zou HF(邹汉法); Jiang GB(江桂斌); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Hu, Ligang,Xu, Songyun,Pan, Chensong,et al. Preparation of a biochip on porous silicon and application for label-free detection of small molecule-protein interactions[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2007,21(7):1277-1281.
APA Hu, Ligang.,Xu, Songyun.,Pan, Chensong.,Zou, Hanfa.,Jiang, Guibin.,...&邹汉法.(2007).Preparation of a biochip on porous silicon and application for label-free detection of small molecule-protein interactions.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,21(7),1277-1281.
MLA Hu, Ligang,et al."Preparation of a biochip on porous silicon and application for label-free detection of small molecule-protein interactions".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 21.7(2007):1277-1281.
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