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题名: Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes
作者: Li, Xin;  Xu, Songyun;  Pan, Chensong;  Zhou, Houjiang;  Jiang, Xiaogang;  Mang, Yu;  Ye, Mingliang;  Zou, Hanfa
通讯作者: 邹汉法
关键词: carbon nanotubes ;  human plasma ;  liquid chromatography ;  mass spectrometry ;  peptidome
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2007-04-01
DOI: 10.1002/jssc.200600479
卷: 30, 期:6, 页:930-943
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Human plasma contains a complex matrix of proteolytically derived peptides (plasma peptidome) that may provide a correlate of biological events occurring in the entire organism. Analyzing these peptides from a small amount of serum/plasma is difficult due to the complexity of the sample and the low levels of these peptides. Here, we describe a novel peptidome analysis approach using multiwalled carbon nanotubes (MWCNTs) as an alternative adsorbent to capture endogenous peptides from human plasma. Harvested peptides were analyzed by using liquid chromatography-mass spectrometry as a means of detecting and assessing the adsorbed molecules. The improved sensitivity and resolution obtained by using liquid chromatography-mass spectrometry allowed detection of 2521 peptide features (m/z 300-1800 range) in about 50 mu L of plasma. 374 unique peptides were identified with high confidence by two-dimensional liquid chromatography system coupled to a nano-spray ionization linear ion trap-mass spectrometer. High recovery of BSA digest peptides enriched with MWCNTs, in both standard buffer and high abundance protein solution, was observed. Comparative studies showed that MWCNTs were superior to C18 and C8 for the capture of the smaller peptides. This approach could hold promise of routine plasma peptidome analysis.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  SOLID-PHASE EXTRACTION ;  LIQUID-CHROMATOGRAPHY ;  HUMAN SERUM ;  PROTEOMIC ANALYSIS ;  REMOVE ALBUMIN ;  PROTEINS ;  CAPTURE ;  MARKERS ;  COLUMN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000246348000019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98545
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Li, Xin,Xu, Songyun,Pan, Chensong,et al. Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(6):930-943.
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