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Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes
Li, Xin; Xu, Songyun; Pan, Chensong; Zhou, Houjiang; Jiang, Xiaogang; Mang, Yu; Ye, Mingliang; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Carbon Nanotubes Human Plasma Liquid Chromatography Mass Spectrometry Peptidome
刊名JOURNAL OF SEPARATION SCIENCE
2007-04-01
ISSN1615-9306
DOI10.1002/jssc.200600479
30期:6页:930-943
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TANDEM MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; LIQUID-CHROMATOGRAPHY ; HUMAN SERUM ; PROTEOMIC ANALYSIS ; REMOVE ALBUMIN ; PROTEINS ; CAPTURE ; MARKERS ; COLUMN
英文摘要Human plasma contains a complex matrix of proteolytically derived peptides (plasma peptidome) that may provide a correlate of biological events occurring in the entire organism. Analyzing these peptides from a small amount of serum/plasma is difficult due to the complexity of the sample and the low levels of these peptides. Here, we describe a novel peptidome analysis approach using multiwalled carbon nanotubes (MWCNTs) as an alternative adsorbent to capture endogenous peptides from human plasma. Harvested peptides were analyzed by using liquid chromatography-mass spectrometry as a means of detecting and assessing the adsorbed molecules. The improved sensitivity and resolution obtained by using liquid chromatography-mass spectrometry allowed detection of 2521 peptide features (m/z 300-1800 range) in about 50 mu L of plasma. 374 unique peptides were identified with high confidence by two-dimensional liquid chromatography system coupled to a nano-spray ionization linear ion trap-mass spectrometer. High recovery of BSA digest peptides enriched with MWCNTs, in both standard buffer and high abundance protein solution, was observed. Comparative studies showed that MWCNTs were superior to C18 and C8 for the capture of the smaller peptides. This approach could hold promise of routine plasma peptidome analysis.
语种英语
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WOS记录号WOS:000246348000019
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被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98545
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Li, Xin,Xu, Songyun,Pan, Chensong,et al. Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(6):930-943.
APA Li, Xin.,Xu, Songyun.,Pan, Chensong.,Zhou, Houjiang.,Jiang, Xiaogang.,...&邹汉法.(2007).Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes.JOURNAL OF SEPARATION SCIENCE,30(6),930-943.
MLA Li, Xin,et al."Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes".JOURNAL OF SEPARATION SCIENCE 30.6(2007):930-943.
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