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Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes
Li, Xin; Xu, Songyun; Pan, Chensong; Zhou, Houjiang; Jiang, Xiaogang; Mang, Yu; Ye, Mingliang; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
KeywordCarbon Nanotubes Human Plasma Liquid Chromatography Mass Spectrometry Peptidome
Source PublicationJOURNAL OF SEPARATION SCIENCE
2007-04-01
ISSN1615-9306
DOI10.1002/jssc.200600479
Volume30Issue:6Pages:930-943
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordTANDEM MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; LIQUID-CHROMATOGRAPHY ; HUMAN SERUM ; PROTEOMIC ANALYSIS ; REMOVE ALBUMIN ; PROTEINS ; CAPTURE ; MARKERS ; COLUMN
AbstractHuman plasma contains a complex matrix of proteolytically derived peptides (plasma peptidome) that may provide a correlate of biological events occurring in the entire organism. Analyzing these peptides from a small amount of serum/plasma is difficult due to the complexity of the sample and the low levels of these peptides. Here, we describe a novel peptidome analysis approach using multiwalled carbon nanotubes (MWCNTs) as an alternative adsorbent to capture endogenous peptides from human plasma. Harvested peptides were analyzed by using liquid chromatography-mass spectrometry as a means of detecting and assessing the adsorbed molecules. The improved sensitivity and resolution obtained by using liquid chromatography-mass spectrometry allowed detection of 2521 peptide features (m/z 300-1800 range) in about 50 mu L of plasma. 374 unique peptides were identified with high confidence by two-dimensional liquid chromatography system coupled to a nano-spray ionization linear ion trap-mass spectrometer. High recovery of BSA digest peptides enriched with MWCNTs, in both standard buffer and high abundance protein solution, was observed. Comparative studies showed that MWCNTs were superior to C18 and C8 for the capture of the smaller peptides. This approach could hold promise of routine plasma peptidome analysis.
Language英语
URL查看原文
WOS IDWOS:000246348000019
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/98545
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Li, Xin,Xu, Songyun,Pan, Chensong,et al. Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(6):930-943.
APA Li, Xin.,Xu, Songyun.,Pan, Chensong.,Zhou, Houjiang.,Jiang, Xiaogang.,...&邹汉法.(2007).Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes.JOURNAL OF SEPARATION SCIENCE,30(6),930-943.
MLA Li, Xin,et al."Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes".JOURNAL OF SEPARATION SCIENCE 30.6(2007):930-943.
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