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Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets
Xu, Junguang; Zhao, Xiaoming; Wang, Xiuli; Zhao, Zongbao; Du, Yuguang; Du YG(杜昱光); Du YG(杜昱光)
关键词Oligochitosan Phytophthora Capsici Mechanism Internalization Clsm
刊名PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
2007-06-01
DOI10.1016/j.pestbp.2006.10.010
88期:2页:167-175
收录类别SCI
文章类型Article
部门归属18
项目归属1805,1816
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Entomology ; Physiology
研究领域[WOS]Biochemistry & Molecular Biology ; Entomology ; Physiology
关键词[WOS]ANTIMICROBIAL PEPTIDE ; CHITOSAN ; CHITOOLIGOSACCHARIDES ; RECEPTOR ; PEA
英文摘要The mechanism of action of oligochitosan, which hasshown great antifungal activity against Phytophthora capsici in vitro, was studied using 2-aminoacridone-labeled oligoclutosan (2-AMAC-oligochitosan) and a gel-retardation experiment. Internalization of 2-AMAC-oligochitosan in cysts, gernitubes and sporangia of P. capsici was confirmed by confocal laser scan microscopy (CLSM), and the degree of uptake depended on the incubation concentration. 2-AMAC-oligochitosan localized mainly in the cytoplasm and showed no binding to both cell wall and cell membrane. Mannose, an inhibitor for oligochitosan uptake by macrophages, could not inhibit the internalization of oligochitosan in P. capsici. The gel-retardation experiment showed that oligochitosan bound strongly to DNA and RNA of P. capsici. These results indicate that oligochitosan exerts its antifungal activity by penetrating the cell membrane and putative binding to intracellular targets such as DNA and RNA. (c) 2006 Elsevier Inc. All rights reserved.
语种英语
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WOS记录号WOS:000246930700008
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98573
专题中国科学院大连化学物理研究所
通讯作者Du YG(杜昱光); Du YG(杜昱光)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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Xu, Junguang,Zhao, Xiaoming,Wang, Xiuli,et al. Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2007,88(2):167-175.
APA Xu, Junguang.,Zhao, Xiaoming.,Wang, Xiuli.,Zhao, Zongbao.,Du, Yuguang.,...&杜昱光.(2007).Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets.PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,88(2),167-175.
MLA Xu, Junguang,et al."Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 88.2(2007):167-175.
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