中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets
作者: Xu, Junguang;  Zhao, Xiaoming;  Wang, Xiuli;  Zhao, Zongbao;  Du, Yuguang
通讯作者: 杜昱光
关键词: oligochitosan ;  Phytophthora capsici ;  mechanism ;  internalization ;  CLSM
刊名: PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
发表日期: 2007-06-01
DOI: 10.1016/j.pestbp.2006.10.010
卷: 88, 期:2, 页:167-175
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1805,1816
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Entomology ;  Physiology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Entomology ;  Physiology
英文摘要: The mechanism of action of oligochitosan, which hasshown great antifungal activity against Phytophthora capsici in vitro, was studied using 2-aminoacridone-labeled oligoclutosan (2-AMAC-oligochitosan) and a gel-retardation experiment. Internalization of 2-AMAC-oligochitosan in cysts, gernitubes and sporangia of P. capsici was confirmed by confocal laser scan microscopy (CLSM), and the degree of uptake depended on the incubation concentration. 2-AMAC-oligochitosan localized mainly in the cytoplasm and showed no binding to both cell wall and cell membrane. Mannose, an inhibitor for oligochitosan uptake by macrophages, could not inhibit the internalization of oligochitosan in P. capsici. The gel-retardation experiment showed that oligochitosan bound strongly to DNA and RNA of P. capsici. These results indicate that oligochitosan exerts its antifungal activity by penetrating the cell membrane and putative binding to intracellular targets such as DNA and RNA. (c) 2006 Elsevier Inc. All rights reserved.
关键词[WOS]: ANTIMICROBIAL PEPTIDE ;  CHITOSAN ;  CHITOOLIGOSACCHARIDES ;  RECEPTOR ;  PEA
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000246930700008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98573
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Xu, Junguang,Zhao, Xiaoming,Wang, Xiuli,et al. Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2007,88(2):167-175.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Xu, Junguang]'s Articles
 [Zhao, Xiaoming]'s Articles
 [Wang, Xiuli]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Xu, Junguang]‘s Articles
 [Zhao, Xiaoming]‘s Articles
 [Wang, Xiuli]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace