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题名: Method development of efficient protein extraction in bone tissue for proteome analysis
作者: Jiang, Xiaogang;  Ye, Mingliang;  Jiang, Xinning;  Liu, Guangpeng;  Feng, Shun;  Cui, Lei;  Zou, Hanfa
通讯作者: 邹汉法
关键词: protein extraction ;  bone proteome ;  shotgun proteomics ;  tandem mass spectrometry ;  bone diseases ;  biomarker discovery
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2007
DOI: 10.1021/pr070056t
卷: 6, 期:6, 页:2287-2294
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Exploring bone proteome is an important and challenging task for understanding the mechanisms of physiological/pathological process of bone tissue. However, classical methods of protein extraction for soft tissues and cells are not applicable for bone tissue. Therefore, method development of efficient protein extraction is critical for bone proteome analysis. We found in this study that the protein extraction efficiency was improved significantly when bone tissue was demineralized by hydrochloric acid (HCl). A sequential protein extraction method was developed for large-scale proteome analysis of bone tissue. The bone tissue was first demineralized by HCl solution and then extracted using three different lysis buffers. As large amounts of acid soluble proteins also presented in the HCl solution, besides collection of proteins in the extracted lysis buffers, the proteins in the demineralized HCl solution were also collected for proteome analysis. Automated 2D-LC-MS/MS analysis of the collected protein fractions resulted in the identification of 6202 unique peptides which matched 2479 unique proteins. The identified proteins revealed a broad diversity in the protein identity and function. More than 40 bone-specific proteins and 15 potential protein biomarkers previously reported were observed in this study. It was demonstrated that the developed extraction method of proteins in bone tissue, which was also the first large-scale proteomic study of bone, was very efficient for comprehensive analysis of bone proteome and might be helpful for clarifying the mechanisms of bone diseases.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  INTEGRAL MEMBRANE-PROTEINS ;  MESENCHYMAL STEM-CELLS ;  RHEUMATOID-ARTHRITIS ;  LIQUID-CHROMATOGRAPHY ;  YEAST PROTEOME ;  OSTEOARTHRITIS ;  EXPRESSION ;  CARTILAGE ;  IDENTIFICATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000246893500025
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98613
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Shanghai Tissue Engn Res & Dev Ctr, Shanghai 200235, Peoples R China
3.Suzhou Univ, Sch Med, Suzhou 215007, Peoples R China

Recommended Citation:
Jiang, Xiaogang,Ye, Mingliang,Jiang, Xinning,et al. Method development of efficient protein extraction in bone tissue for proteome analysis[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(6):2287-2294.
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