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题名: Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines
作者: Su, Xingye;  Hu, Lianghai;  Kong, Liang;  Lei, Xiaoyuan;  Zou, Hanfa
通讯作者: 邹汉法
关键词: affinity chromatography ;  biological fingerprinting analysis ;  immobilized DNA stationary phase ;  traditional Chinese medicines
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2007-06-22
DOI: 10.1016/j.chroma.2007.03.050
卷: 1154, 期:1-2, 页:132-137
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A stationary phase for high performance affinity chromatography with immobilization of DNA onto silica gel was prepared and characterized. The effect of the ionic strength, concentration of Mg2+, EDTA and CH3 CN in the mobile phase on the retention of alkaloids were investigated. With this stationary phase, biological fingerprinting analysis of traditional Chinese medicines (TCMs) Coptis chinensis Franch and Rheum palmatum L. was performed with both one-dimensional (1-D) and two-dimensional (2-D) chromatography. The 1-D chromatography was performed with isocratic and gradient elution and 2-D chromatography was developed with immobilized DNA column combined with silica monolithic ODS column. It was found that 7 compounds in Coptis chinensis Franch including berberine, palmatine and jatrorrhizine, 14 compounds in Rheum palmatum L. including aloe-emodin, rhein, emodin, chrysophannol-8-O-glucophranoside and physionl-8-O-glucophranoside were active in binding to the immobilized DNA. (c) 2007 Elsevier B.V All rights reserved.
关键词[WOS]: L-RNA APTAMER ;  BIOACTIVE COMPOUNDS ;  ANGELICA-SINENSIS ;  IN-VITRO ;  BINDING ;  TARGET ;  PURIFICATION ;  SPECTROMETRY ;  SELECTIVITY ;  SEPARATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000247419300016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98615
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Su, Xingye,Hu, Lianghai,Kong, Liang,et al. Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines[J]. JOURNAL OF CHROMATOGRAPHY A,2007,1154(1-2):132-137.
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