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题名: Highly specific enrichment of phosphopeptides by zirconium dioxide nanoparticles for phosphoproteome analysis
作者: Zhou, Houjiang;  Tian, Ruijun;  Ye, Mingliang;  Xu, Songyun;  Feng, Shun;  Pan, Chensong;  Jiang, Xiaogang;  Li, Xin;  Zou, Hanfa
通讯作者: 邹汉法
关键词: MALDI-TOF MS ;  nano-LC MS/MS ;  phosphopeptides ;  phosphoproteome analysis ;  zirconium dioxide nanoparticles
刊名: ELECTROPHORESIS
发表日期: 2007-07-01
DOI: 10.1002/elps.200600718
卷: 28, 期:13, 页:2201-2215
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Large-scale characterization of phosphoproteins requires highly specific methods for the purification of phosphopeptides because of the low abundance of phosphoproteins and substoichiometry of phosphorylation. A phosphopeptide enrichment method using ZrO2 nanoparticles is presented. The high specificity of this approach was demonstrated by the isolation of phosphopeptides from the digests of model phosphoproteins. The strong affinity of ZrO2 nanoparticles to phosphopeptides enables the specific enrichment of phosphopeptides from a complex peptide mixture in which the abundance of phosphopeptides is two orders of magnitude lower than that of nonphosphopeptides. Superior selectivity of ZrO2 nanoparticles for the enrichment of phosphorylated peptides than that of conventional immobilized metal affinity chromatography was observed. Femtomole phosphopeptides from digestion products could be enriched by ZrO2 nanoparticles and can be well detected by MALDI mass spectrometric analysis. ZrO2 nanoparticles were further applied to selectively isolate phosphopeptides from the tryptic digestion of mouse liver lysate for phosphoproteome analysis by nanoliter LC MS/MS (nano-LC-MS/MS) and MS/MS/MS. A total of 248 defining phosphorylation sites and 140 phosphorylated peptides were identified by manual validation using a series of rigid criteria.
关键词[WOS]: MASS-SPECTROMETRY ;  AFFINITY-CHROMATOGRAPHY ;  PHOSPHORYLATED PROTEINS ;  TYROSINE PHOSPHORYLATION ;  POSTTRANSLATIONAL MODIFICATIONS ;  SURFACE-PROPERTIES ;  PROTEOMIC ANALYSIS ;  MS ANALYSIS ;  LC-MS/MS ;  IDENTIFICATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000248190400010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98655
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Zhou, Houjiang,Tian, Ruijun,Ye, Mingliang,et al. Highly specific enrichment of phosphopeptides by zirconium dioxide nanoparticles for phosphoproteome analysis[J]. ELECTROPHORESIS,2007,28(13):2201-2215.
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