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题名: Preparation and evaluation of a vancomycin-immobilized silica monolith as chiral stationary phase for CEC
作者: Dong, Xiaoli;  Dong, Jing;  Ou, Junjie;  Zhu, Yan;  Zou, Hanfa
通讯作者: 邹汉法
关键词: CEC ;  enantioseparation ;  silica monolith ;  vancomycin
刊名: ELECTROPHORESIS
发表日期: 2007-08-01
DOI: 10.1002/elps.200600605
卷: 28, 期:15, 页:2606-2612
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 2;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Enantiomeric separations in CEC with the macrocyclic antibiotic vancomycin immobilized silica monolith as a chiral stationary phase are presented. The monolithic silica capillary columns were prepared by a sol-gel process in fused-silica capillaries with an inner diameter of 50 mu m and subsequently in situ immobilization of vancomycin as a chiral selector by reductive amination. Enantioselectivity was obtained for eight pairs of enantiomers in nonaqueous polar organic or aqueous mobile phases and most of them were baseline-separated with high column efficiencies. it was observed that the organic modifier ratio (MeOH/ACN) in the polar organic mobile phase played a significant role in controlling the resolution and efficiency of the enantiomers. In enantiomeric separation of propranolol, repeatability for column efficiency and resolution in the nonaqueous mobile phase was given in terms of RSD values at 1.1 and 2.3% (n = 5) for run-to-run injections and 7.2 and 9.6% (n = 5) for column-to-column testing while repeatability for the separation of thalidomide in the aqueous mobile phase was given in terms of RSD values at 1.5, 2.8% and 6.1, 10.5%, respectively.
关键词[WOS]: TUBULAR CAPILLARY ELECTROCHROMATOGRAPHY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  CHARGED POLYACRYLAMIDE-GELS ;  ENANTIOMERIC SEPARATIONS ;  MASS SPECTROMETRY ;  COLUMN ;  ENANTIOSEPARATION ;  ELECTROPHORESIS ;  DERIVATIVES ;  RESOLUTION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000248938400008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98673
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Zhejiang Univ, Dept Chem, Hangzhou 310027, Peoples R China

Recommended Citation:
Dong, Xiaoli,Dong, Jing,Ou, Junjie,et al. Preparation and evaluation of a vancomycin-immobilized silica monolith as chiral stationary phase for CEC[J]. ELECTROPHORESIS,2007,28(15):2606-2612.
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