DICP OpenIR
Preparation and evaluation of a vancomycin-immobilized silica monolith as chiral stationary phase for CEC
Dong, Xiaoli; Dong, Jing; Ou, Junjie; Zhu, Yan; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
KeywordCec Enantioseparation Silica Monolith Vancomycin
Source PublicationELECTROPHORESIS
2007-08-01
ISSN0173-0835
DOI10.1002/elps.200600605
Volume28Issue:15Pages:2606-2612
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank2;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordTUBULAR CAPILLARY ELECTROCHROMATOGRAPHY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; CHARGED POLYACRYLAMIDE-GELS ; ENANTIOMERIC SEPARATIONS ; MASS SPECTROMETRY ; COLUMN ; ENANTIOSEPARATION ; ELECTROPHORESIS ; DERIVATIVES ; RESOLUTION
AbstractEnantiomeric separations in CEC with the macrocyclic antibiotic vancomycin immobilized silica monolith as a chiral stationary phase are presented. The monolithic silica capillary columns were prepared by a sol-gel process in fused-silica capillaries with an inner diameter of 50 mu m and subsequently in situ immobilization of vancomycin as a chiral selector by reductive amination. Enantioselectivity was obtained for eight pairs of enantiomers in nonaqueous polar organic or aqueous mobile phases and most of them were baseline-separated with high column efficiencies. it was observed that the organic modifier ratio (MeOH/ACN) in the polar organic mobile phase played a significant role in controlling the resolution and efficiency of the enantiomers. In enantiomeric separation of propranolol, repeatability for column efficiency and resolution in the nonaqueous mobile phase was given in terms of RSD values at 1.1 and 2.3% (n = 5) for run-to-run injections and 7.2 and 9.6% (n = 5) for column-to-column testing while repeatability for the separation of thalidomide in the aqueous mobile phase was given in terms of RSD values at 1.5, 2.8% and 6.1, 10.5%, respectively.
Language英语
URL查看原文
WOS IDWOS:000248938400008
Citation statistics
Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/98673
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Zhejiang Univ, Dept Chem, Hangzhou 310027, Peoples R China
Recommended Citation
GB/T 7714
Dong, Xiaoli,Dong, Jing,Ou, Junjie,et al. Preparation and evaluation of a vancomycin-immobilized silica monolith as chiral stationary phase for CEC[J]. ELECTROPHORESIS,2007,28(15):2606-2612.
APA Dong, Xiaoli.,Dong, Jing.,Ou, Junjie.,Zhu, Yan.,Zou, Hanfa.,...&邹汉法.(2007).Preparation and evaluation of a vancomycin-immobilized silica monolith as chiral stationary phase for CEC.ELECTROPHORESIS,28(15),2606-2612.
MLA Dong, Xiaoli,et al."Preparation and evaluation of a vancomycin-immobilized silica monolith as chiral stationary phase for CEC".ELECTROPHORESIS 28.15(2007):2606-2612.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Dong, Xiaoli]'s Articles
[Dong, Jing]'s Articles
[Ou, Junjie]'s Articles
Baidu academic
Similar articles in Baidu academic
[Dong, Xiaoli]'s Articles
[Dong, Jing]'s Articles
[Ou, Junjie]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Dong, Xiaoli]'s Articles
[Dong, Jing]'s Articles
[Ou, Junjie]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.