DICP OpenIR
Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device
Ye, Nannan; Qin, Jianhua; Shi, Weiwei; Lin, Bingcheng; Lin BC(林炳承); Lin BC(林炳承)
KeywordApoptosis Doxorubicin Human Hepatocellular Carcinoma Cells Microfluidic Device
Source PublicationELECTROPHORESIS
2007-04-01
DOI10.1002/elps.200600450
Volume28Issue:7Pages:1146-1153
Indexed BySCI
SubtypeArticle
Department18
Funding Project1807
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordTOTAL ANALYSIS SYSTEMS ; DIFFERENTIATION ; GENERATION ; GRADIENTS ; HALLMARKS ; DEATH ; FLOW
AbstractApoptosis has now established its importance in numerous areas of biology and is recently receiving great attention as an important topic related to the development of diseases. In this work, an integrated microfluidic device was developed to characterize doxorubicin-induced apoptosis in human hepatocellular carcinoma (HepG2) cells. A continuous concentration gradient of stimulator (doxorubicin) was generated in the upstream network and used to perfuse downstream cultured HepG2 cells. The appropriate fluorescent dyes were introduced into cells from the inlets connected to the cell culture chambers, allowing one to distinguish apoptotic cells from nonapoptotic or necrotic cells. The resultant fluorescence of cellular population was monitored and quantified with single-cell resolution to infer the apoptosis process being studied. The feasibility of studying apoptosis was demonstrated by measuring several apoptotic events, including morphological alterations, plasma membrane phosphatidylserine externalization, and mitochondrial membrane potential collapse. This microfluidic device, integrating the cell culture, stimulation, staining, and washing steps into a single device, can simultaneously generate a number of experimental conditions and investigate multiple parameters relating stimulation to apoptosis. It offers a unique platform to characterize various cellular responses in a high-throughput fashion, which is otherwise impossible with conventional methods.
Language英语
URL查看原文
WOS IDWOS:000245682000015
Citation statistics
Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/98693
Collection中国科学院大连化学物理研究所
Corresponding AuthorLin BC(林炳承); Lin BC(林炳承)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Ye, Nannan,Qin, Jianhua,Shi, Weiwei,et al. Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device[J]. ELECTROPHORESIS,2007,28(7):1146-1153.
APA Ye, Nannan,Qin, Jianhua,Shi, Weiwei,Lin, Bingcheng,林炳承,&林炳承.(2007).Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device.ELECTROPHORESIS,28(7),1146-1153.
MLA Ye, Nannan,et al."Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device".ELECTROPHORESIS 28.7(2007):1146-1153.
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