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Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device
Ye, Nannan; Qin, Jianhua; Shi, Weiwei; Lin, Bingcheng; Lin BC(林炳承); Lin BC(林炳承)
关键词Apoptosis Doxorubicin Human Hepatocellular Carcinoma Cells Microfluidic Device
刊名ELECTROPHORESIS
2007-04-01
DOI10.1002/elps.200600450
28期:7页:1146-1153
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TOTAL ANALYSIS SYSTEMS ; DIFFERENTIATION ; GENERATION ; GRADIENTS ; HALLMARKS ; DEATH ; FLOW
英文摘要Apoptosis has now established its importance in numerous areas of biology and is recently receiving great attention as an important topic related to the development of diseases. In this work, an integrated microfluidic device was developed to characterize doxorubicin-induced apoptosis in human hepatocellular carcinoma (HepG2) cells. A continuous concentration gradient of stimulator (doxorubicin) was generated in the upstream network and used to perfuse downstream cultured HepG2 cells. The appropriate fluorescent dyes were introduced into cells from the inlets connected to the cell culture chambers, allowing one to distinguish apoptotic cells from nonapoptotic or necrotic cells. The resultant fluorescence of cellular population was monitored and quantified with single-cell resolution to infer the apoptosis process being studied. The feasibility of studying apoptosis was demonstrated by measuring several apoptotic events, including morphological alterations, plasma membrane phosphatidylserine externalization, and mitochondrial membrane potential collapse. This microfluidic device, integrating the cell culture, stimulation, staining, and washing steps into a single device, can simultaneously generate a number of experimental conditions and investigate multiple parameters relating stimulation to apoptosis. It offers a unique platform to characterize various cellular responses in a high-throughput fashion, which is otherwise impossible with conventional methods.
语种英语
原文出处查看原文
WOS记录号WOS:000245682000015
引用统计
被引频次:39[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98693
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承); Lin BC(林炳承)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
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Ye, Nannan,Qin, Jianhua,Shi, Weiwei,et al. Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device[J]. ELECTROPHORESIS,2007,28(7):1146-1153.
APA Ye, Nannan,Qin, Jianhua,Shi, Weiwei,Lin, Bingcheng,林炳承,&林炳承.(2007).Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device.ELECTROPHORESIS,28(7),1146-1153.
MLA Ye, Nannan,et al."Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device".ELECTROPHORESIS 28.7(2007):1146-1153.
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