中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device
作者: Ye, Nannan;  Qin, Jianhua;  Shi, Weiwei;  Lin, Bingcheng
通讯作者: 林炳承
关键词: apoptosis ;  doxorubicin ;  human hepatocellular carcinoma cells ;  microfluidic device
刊名: ELECTROPHORESIS
发表日期: 2007-04-01
DOI: 10.1002/elps.200600450
卷: 28, 期:7, 页:1146-1153
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Apoptosis has now established its importance in numerous areas of biology and is recently receiving great attention as an important topic related to the development of diseases. In this work, an integrated microfluidic device was developed to characterize doxorubicin-induced apoptosis in human hepatocellular carcinoma (HepG2) cells. A continuous concentration gradient of stimulator (doxorubicin) was generated in the upstream network and used to perfuse downstream cultured HepG2 cells. The appropriate fluorescent dyes were introduced into cells from the inlets connected to the cell culture chambers, allowing one to distinguish apoptotic cells from nonapoptotic or necrotic cells. The resultant fluorescence of cellular population was monitored and quantified with single-cell resolution to infer the apoptosis process being studied. The feasibility of studying apoptosis was demonstrated by measuring several apoptotic events, including morphological alterations, plasma membrane phosphatidylserine externalization, and mitochondrial membrane potential collapse. This microfluidic device, integrating the cell culture, stimulation, staining, and washing steps into a single device, can simultaneously generate a number of experimental conditions and investigate multiple parameters relating stimulation to apoptosis. It offers a unique platform to characterize various cellular responses in a high-throughput fashion, which is otherwise impossible with conventional methods.
关键词[WOS]: TOTAL ANALYSIS SYSTEMS ;  DIFFERENTIATION ;  GENERATION ;  GRADIENTS ;  HALLMARKS ;  DEATH ;  FLOW
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000245682000015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98693
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China

Recommended Citation:
Ye, Nannan,Qin, Jianhua,Shi, Weiwei,et al. Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device[J]. ELECTROPHORESIS,2007,28(7):1146-1153.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Ye, Nannan]'s Articles
 [Qin, Jianhua]'s Articles
 [Shi, Weiwei]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Ye, Nannan]‘s Articles
 [Qin, Jianhua]‘s Articles
 [Shi, Weiwei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace