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Fabrication of two-weir structure-based packed columns for on-chip solid-phase extraction of DNA
Zhong, Runtao; Liu, Dayu; Yu, Linfen; Ye, Nannan; Dai, Zhongpeng; Oin, Jianhua; Lin, Bingcheng; Lin BC(林炳承); Lin BC(林炳承)
关键词Dna Purification Microfabrication Packed Column Solid-phase Extraction Two-weir Structure
刊名ELECTROPHORESIS
2007-08-01
DOI10.1002/elps.200600604
28期:16页:2920-2926
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]POLYMERASE-CHAIN-REACTION ; MICROFLUIDIC CHIPS ; SAMPLE PREPARATION ; NUCLEIC-ACIDS ; MICROCHIP ; PURIFICATION ; SYSTEM ; DEVICE ; PRECONCENTRATION ; AMPLIFICATION
英文摘要Microchip-based packed column SPE of DNA was performed using the microfabricated two-weir structure within a microchannel. We developed two methods to fabricate the two-weir structured glass chips: a "two-side etching/alignment" method and a simplified "one-side/two-step etching" method. The former rnethod required a straightforward alignment step, while the latter approach comprised a simplified wet etching process using paraffin wax as the temporary protective layer. Both methods were convenient and rapid as compared to the previous approaches. Through a reversibly sealed bead-introduction channel, beads can be fed into or out of the chip columns, thus enabling refreshment of the packing materials. Using the proposed chip columns, highly efficient,lambda-DNA extractions (average recovery > 80%) were performed with good chip-to-chip reproducibility (RSD < 10%). The on-chip SPE procedure was completed within 15 min at the flow rate of 3 mu L/min and the bulk of the loaded DNA was eluted within a small volume of similar to 8 mu L. Application of the microchip-based packed columns was demonstrated by purifying PCR-amplifiable genomic DNA from human hepatocellular carcinoma (HepG2) cells and human whole blood samples.
语种英语
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WOS记录号WOS:000249286500018
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98699
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承); Lin BC(林炳承)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Zhong, Runtao,Liu, Dayu,Yu, Linfen,et al. Fabrication of two-weir structure-based packed columns for on-chip solid-phase extraction of DNA[J]. ELECTROPHORESIS,2007,28(16):2920-2926.
APA Zhong, Runtao.,Liu, Dayu.,Yu, Linfen.,Ye, Nannan.,Dai, Zhongpeng.,...&林炳承.(2007).Fabrication of two-weir structure-based packed columns for on-chip solid-phase extraction of DNA.ELECTROPHORESIS,28(16),2920-2926.
MLA Zhong, Runtao,et al."Fabrication of two-weir structure-based packed columns for on-chip solid-phase extraction of DNA".ELECTROPHORESIS 28.16(2007):2920-2926.
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