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题名: Fabrication of two-weir structure-based packed columns for on-chip solid-phase extraction of DNA
作者: Zhong, Runtao;  Liu, Dayu;  Yu, Linfen;  Ye, Nannan;  Dai, Zhongpeng;  Oin, Jianhua;  Lin, Bingcheng
通讯作者: 林炳承
关键词: DNA purification ;  microfabrication ;  packed column ;  solid-phase extraction ;  two-weir structure
刊名: ELECTROPHORESIS
发表日期: 2007-08-01
DOI: 10.1002/elps.200600604
卷: 28, 期:16, 页:2920-2926
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Microchip-based packed column SPE of DNA was performed using the microfabricated two-weir structure within a microchannel. We developed two methods to fabricate the two-weir structured glass chips: a "two-side etching/alignment" method and a simplified "one-side/two-step etching" method. The former rnethod required a straightforward alignment step, while the latter approach comprised a simplified wet etching process using paraffin wax as the temporary protective layer. Both methods were convenient and rapid as compared to the previous approaches. Through a reversibly sealed bead-introduction channel, beads can be fed into or out of the chip columns, thus enabling refreshment of the packing materials. Using the proposed chip columns, highly efficient,lambda-DNA extractions (average recovery > 80%) were performed with good chip-to-chip reproducibility (RSD < 10%). The on-chip SPE procedure was completed within 15 min at the flow rate of 3 mu L/min and the bulk of the loaded DNA was eluted within a small volume of similar to 8 mu L. Application of the microchip-based packed columns was demonstrated by purifying PCR-amplifiable genomic DNA from human hepatocellular carcinoma (HepG2) cells and human whole blood samples.
关键词[WOS]: POLYMERASE-CHAIN-REACTION ;  MICROFLUIDIC CHIPS ;  SAMPLE PREPARATION ;  NUCLEIC-ACIDS ;  MICROCHIP ;  PURIFICATION ;  SYSTEM ;  DEVICE ;  PRECONCENTRATION ;  AMPLIFICATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000249286500018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98699
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zhong, Runtao,Liu, Dayu,Yu, Linfen,et al. Fabrication of two-weir structure-based packed columns for on-chip solid-phase extraction of DNA[J]. ELECTROPHORESIS,2007,28(16):2920-2926.
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