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Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis
Shen, Zheng; Liu, Xiaojun; Zhou, Xiaomian; Liang, Aiye; Wu, Dapeng; Yu, Linfen; Dai, Zhongpeng; Qin, Jianhua; Lin, Bingcheng; Lin BC(林炳承); Lin BC(林炳承)
KeywordCapillary Array Electrophoresis Dna Interaction Microfluidic Chip Netropsin
Source PublicationJOURNAL OF SEPARATION SCIENCE
2007-07-01
DOI10.1002/jssc.200600530
Volume30Issue:10Pages:1544-1548
Indexed BySCI
SubtypeArticle
Department18
Funding Project1807
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordIONIZATION MASS-SPECTROMETRY ; DRUG-DNA INTERACTIONS ; ZONE-ELECTROPHORESIS ; ANALYSIS SYSTEMS ; AFFINITY ; SEQUENCE ; CHIP ; SELECTIVITY ; COMPLEXES
AbstractMicrofabricated capillary array electrophoresis (mu-CAE) was applied to study the interaction between minor groove binder netropsin and a non-selfcomplementary 12 mer double stranded oligodeoxynucleotide: d(CCCCTATACCGC) center dot d(GCGGTATAGGGG). ESI-MS was used to provide an independent verification of the microchip electrophoresis derived data. Simultaneous parallel quantitative assay of multiple samples was performed in a single run (< 50 s) on the self-developed mu-CAE device. The binding constant and stoichiometry calculated from Scatchard plot were (2.88 +/- 0.23) x 10(5) M-1 and 1:1, respectively. The values showed a good quantitative agreement with the results determined by ESI-MS and those using other methods reported in the literature.
Language英语
URL查看原文
WOS IDWOS:000248240000018
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/98701
Collection中国科学院大连化学物理研究所
Corresponding AuthorLin BC(林炳承); Lin BC(林炳承)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
3.Guangzhou Med Coll, Dept Clin Lab, Guangzhou Municipal Peoples Hosp 1, Guangzhou, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Shen, Zheng,Liu, Xiaojun,Zhou, Xiaomian,et al. Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(10):1544-1548.
APA Shen, Zheng.,Liu, Xiaojun.,Zhou, Xiaomian.,Liang, Aiye.,Wu, Dapeng.,...&林炳承.(2007).Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis.JOURNAL OF SEPARATION SCIENCE,30(10),1544-1548.
MLA Shen, Zheng,et al."Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis".JOURNAL OF SEPARATION SCIENCE 30.10(2007):1544-1548.
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