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Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis
Shen, Zheng; Liu, Xiaojun; Zhou, Xiaomian; Liang, Aiye; Wu, Dapeng; Yu, Linfen; Dai, Zhongpeng; Qin, Jianhua; Lin, Bingcheng; Lin BC(林炳承); Lin BC(林炳承)
关键词Capillary Array Electrophoresis Dna Interaction Microfluidic Chip Netropsin
刊名JOURNAL OF SEPARATION SCIENCE
2007-07-01
DOI10.1002/jssc.200600530
30期:10页:1544-1548
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]IONIZATION MASS-SPECTROMETRY ; DRUG-DNA INTERACTIONS ; ZONE-ELECTROPHORESIS ; ANALYSIS SYSTEMS ; AFFINITY ; SEQUENCE ; CHIP ; SELECTIVITY ; COMPLEXES
英文摘要Microfabricated capillary array electrophoresis (mu-CAE) was applied to study the interaction between minor groove binder netropsin and a non-selfcomplementary 12 mer double stranded oligodeoxynucleotide: d(CCCCTATACCGC) center dot d(GCGGTATAGGGG). ESI-MS was used to provide an independent verification of the microchip electrophoresis derived data. Simultaneous parallel quantitative assay of multiple samples was performed in a single run (< 50 s) on the self-developed mu-CAE device. The binding constant and stoichiometry calculated from Scatchard plot were (2.88 +/- 0.23) x 10(5) M-1 and 1:1, respectively. The values showed a good quantitative agreement with the results determined by ESI-MS and those using other methods reported in the literature.
语种英语
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WOS记录号WOS:000248240000018
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98701
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承); Lin BC(林炳承)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
3.Guangzhou Med Coll, Dept Clin Lab, Guangzhou Municipal Peoples Hosp 1, Guangzhou, Guangdong, Peoples R China
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Shen, Zheng,Liu, Xiaojun,Zhou, Xiaomian,et al. Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(10):1544-1548.
APA Shen, Zheng.,Liu, Xiaojun.,Zhou, Xiaomian.,Liang, Aiye.,Wu, Dapeng.,...&林炳承.(2007).Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis.JOURNAL OF SEPARATION SCIENCE,30(10),1544-1548.
MLA Shen, Zheng,et al."Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis".JOURNAL OF SEPARATION SCIENCE 30.10(2007):1544-1548.
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