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题名: Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis
作者: Shen, Zheng;  Liu, Xiaojun;  Zhou, Xiaomian;  Liang, Aiye;  Wu, Dapeng;  Yu, Linfen;  Dai, Zhongpeng;  Qin, Jianhua;  Lin, Bingcheng
通讯作者: 林炳承
关键词: capillary array electrophoresis ;  DNA ;  interaction ;  microfluidic chip ;  netropsin
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2007-07-01
DOI: 10.1002/jssc.200600530
卷: 30, 期:10, 页:1544-1548
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Microfabricated capillary array electrophoresis (mu-CAE) was applied to study the interaction between minor groove binder netropsin and a non-selfcomplementary 12 mer double stranded oligodeoxynucleotide: d(CCCCTATACCGC) center dot d(GCGGTATAGGGG). ESI-MS was used to provide an independent verification of the microchip electrophoresis derived data. Simultaneous parallel quantitative assay of multiple samples was performed in a single run (< 50 s) on the self-developed mu-CAE device. The binding constant and stoichiometry calculated from Scatchard plot were (2.88 +/- 0.23) x 10(5) M-1 and 1:1, respectively. The values showed a good quantitative agreement with the results determined by ESI-MS and those using other methods reported in the literature.
关键词[WOS]: IONIZATION MASS-SPECTROMETRY ;  DRUG-DNA INTERACTIONS ;  ZONE-ELECTROPHORESIS ;  ANALYSIS SYSTEMS ;  AFFINITY ;  SEQUENCE ;  CHIP ;  SELECTIVITY ;  COMPLEXES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000248240000018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98701
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
3.Guangzhou Med Coll, Dept Clin Lab, Guangzhou Municipal Peoples Hosp 1, Guangzhou, Guangdong, Peoples R China

Recommended Citation:
Shen, Zheng,Liu, Xiaojun,Zhou, Xiaomian,et al. Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(10):1544-1548.
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